Figure 4.

Huh7.5-SEC14L2 cells were transiently transfected (by electroporation) with luciferase containing HCV (S52-replicon) with Wt, 150V, and 206E, individually and in combination. Cells were treated with SOF for 72 hours and HCV replication was measured using a luciferase assay and normalized to a sample 4 hours post electroporation for each construct. Panels A–C indicate the effect of the polymorphisms (A150V and K206E) from patients 10–14 on SOF sensitivity. IC₅₀ values with 95% CIs were calculated by determining the drug concentration that affected a 50% reduction in replication. Mean values per drug concentration are plotted ± SEM. The above is representative of at least 3 independent experiments.