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. 2007 May 1;6(3):383–388. doi: 10.1111/j.1582-4934.2002.tb00516.x

Angiotensinogen gene M235T variant and pre‐eclampsia in Romanian pregnant women

Lucia Procopciuc 1,✉, Gh Jebeleanu 1, I Surcel 2,1, M Puscas 2,1
PMCID: PMC6740124  PMID: 12417054

Abstract

Background. Association between the human angiotensinogen gene and essential hypertension has been confirmed in recent studies. Pre‐eclampsia is a complication of pregnancy characterised by increased vascular resistance, high blood pressure, proteinuria and oedema, that appears in the second and third trimester of pregnancy. The aim of our study was the analysis of M235T mutation in the gene encoding angiotensinogen in Romanian women with different forms of hypertension during pregnancy. Methods. Fourteen women with obstetric complications were tested for M235T angiotensinogen gene mutation. Indications for testing were: severe or mild pre‐eclampsia and pre‐eclampsia associated with chronic hypertension. We also tested for control 6 healthy women. The M235T angiotensinogen gene mutation was analysed by polymerase chain reaction followed by enzymatic digestion with Tth 111I restriction endonuclease enzyme and agarose gel electrophoresis of the products. Results. Eleven (78.57%) of the 14 women with complications of pregnancy had M235T mutation: 9 (64.28%) were found to be heterozygous carriers of the M235T variant of the angiotensinogen gene and 2(14.28%) were found to be homozygous carriers. In the group of women with normal pregnancy, 3 (50%) of the 6 women had M235T mutation: 2 (33.33%) were found to be heterozygous carriers of the M235T variant of the angiotensinogen gene and 1 (16.66%) was found to be homozygous carrier. Conclusions. Our study shows that the M235T variant in the gene encoding angiotensinogen could be a risk factor in mild and severe pre‐eclampsia.

Keywords: arterial hypertension, pre‐eclampsia, pregnancy, angiotensinogen gene, M235T variant, polymerase chain reaction

References

  • 1. Jeunemaitre X., Soubrier F., Kotelevtsev YV., Molecular basis of human hypertension: role of angiotensinogen. Cell, 71: 169–180, 1992. [DOI] [PubMed] [Google Scholar]
  • 2. Laragh J.H., The pill, hypertension, and the toxemias of pregnancy. Am. J. Obstet. Gynecol., 109: 210–213, 1971. [DOI] [PubMed] [Google Scholar]
  • 3. Chesley L.C., Lindheimer M.D., Renal hemodynamics and intravascular volume in normal and hypertensive pregnancy In Handbook of Hypertension, ed. Rubin P.C., vol. 10, Elsevier, Amsterdam , 1988, pp. 38–65. [Google Scholar]
  • 4. Wallenburg H.C.S., Hemodynamics in hypertensive pregnancy In Handbook of Hypertension, ed. Rubin P.C., vol. 10, Elsevier, Amsterdam , 1988, pp. 66–101. [Google Scholar]
  • 5. August P., Sealey J.E., The renin‐ angiotensin system in normal and hypertensive pregnancy and in ovarian function In Hypertension: Pathophysiology, diagnosis, and management, eds. Laragh J.H., Brenner B.M., Raven, New York , 1990, pp. 1761–1778. [Google Scholar]
  • 6. Wilton A.N., Cooper D.W., Brennecke S.P., Bishop S., Marshall P., Absence of close linkage between maternal genes for susceptibility to pre‐eclampsia/eclampsia and HLA DRb. Lancet, 336: 653–657, 1990. [DOI] [PubMed] [Google Scholar]
  • 7. Cooper D.W., Brennecke S.P., Wilton A.N., Genetics of pre‐eclampsia. Hypertension in pregnancy, 12: 1–23, 1990. [Google Scholar]
  • 8. Harrison G.A., Humphrey K.E., Jones N., Badenhop R., Guo G., Elekis G., A genome‐wide linkage study of pre‐eclampsia/eclampsia reveals evidence for a candidate region on 4q. Am. J. Hum. Genet., 60: 1158–1167, 1997. [PMC free article] [PubMed] [Google Scholar]
  • 9. Guo G., Wilton A.N., Fu Y., Qiu H., Brennecke S.P., Cooper D.W., Angiotensinogen gene variation in a population case‐control study of pre‐ecalmsia in Australians and Chinese. Electrophoresis, 18: 1646–1649, 1997. [DOI] [PubMed] [Google Scholar]
  • 10. Department of health (1996), Report on confidential enquires into maternal deaths in the United Kingdom, HMSO, London , 1991–1993. [Google Scholar]
  • 11. Roberts J.M., Taylor R.N., Musici T.J., Rodgers G.M., Hubel C.A., McLaughlin M.K., Preeclampsia: an endothelial cell disorder. Am. J. Obstet. Gynecol., 161: 1200–1204, 1989. [DOI] [PubMed] [Google Scholar]
  • 12. Salafia C.M., Pezzullo J.C., Lopez‐Zeno J.A., Simmens S., Minior V.K., Vintzileos A.M., Placental pathologic features of preterm preeclampsia. Am. J. Obstet. Gynecol., 173: 1097–1105, 1995. [DOI] [PubMed] [Google Scholar]
  • 13. Shanklin D.R., Sibai B.M., Ultrastructural aspects of preeclampsia. Am. J. Obstet. Gynecol., 161: 735–741, 1989. [DOI] [PubMed] [Google Scholar]
  • 14. Keneally J., Pre‐eclampsia: Pathophysiology and Intensive care management, Australasian Anaesthesia, 1–10, 1998.
  • 15. Kupferminc M.J., Peri H., Zwang E., Yaron Y., Wolman I., Eldor A., High prevalence of the prothrombin gene mutation in women with intrauterine growth retardation, abruption placentae and second trimester loss. Acta Obstet. Gynecol. Scand., 79: 963–967, 2000. [PubMed] [Google Scholar]
  • 16. Tewksbury D.A., Dart R.A., High molecular weight angiotensinogen levels in hypertensive pregnant women. Hypertension, 4: 729–734, 1982. [DOI] [PubMed] [Google Scholar]
  • 17. Russ A.P., Maerz W., Ruzicka V., Stein U., Gross W., Rapid detection of the hypertension‐ associated Met 235 Thr allele of the human angiotensinogen gene. Hum. Mol. Genet., 2: 609–610, 1993. [DOI] [PubMed] [Google Scholar]
  • 18. Jeunemaitre X., Charru A., Chattelier G., M235T variant of the human angiotensinogen gene in unselected hypertensive patients. J. Hypertens., 11(suppl. 5):80–81, 1993. [PubMed] [Google Scholar]
  • 19. Ward K., Hata A., Jeunemaitre X., Helin C., Nelson L., Namikawa C., Farrington P.F., Ogasawara M., Suzumori K., Tomoda S., Berrebi S., Sasaki M., Corvol P., Lifton R.P., Lalouel J.M., A molecular variant of angiotensinogen associated with preeclampsia. Nature Genetics, 4: 59–61, 1993. [DOI] [PubMed] [Google Scholar]
  • 20. Moses E.K., Lade J.A., Guo G., Wilton A.I.N., Grehan M., Freed K., Borg A., Terwilliger R., North R., Cooper D.W., Brennecke S.P., A genome scan in families from Australia and New Zealand confirms the presence of a maternal susceptibility locus for pre‐eclampsia, on chromosome 2. Am. J. Hum. Genet., 67: 1581–1585, 2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21. Lahiri D. K., Nurnberger J. I., A rapid non‐ enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucleic Acids Res., 19: 5444, 1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22. Quershi A.I., Frankel M.R., Ottenlips J.R., Stern B.J., Cerebral hemodynamics in preeclampsia and eclampsia. Arch. Neurol., 53: 1226–1231, 1996. [DOI] [PubMed] [Google Scholar]
  • 23. Serra‐Serra V Kyle, P.M. , Chandran R., Redman C.W., The effect of nifedipine and methyldopa on maternal cerebral circulation. Br. J. Obstet. Gynaecol., 104: 532–537, 1997. [DOI] [PubMed] [Google Scholar]

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