Figure 5.

FG Directly Activates Phagocytosis in Mφs

(A) IF images of tumor-phagocytic Mφs. Tumor sections were stained with anti-GFP antibody (green) and anti-F4/80 antibody (red). Arrowheads indicate GFP⁺F4/80⁺ cells. Scale bar, 20 μm.

(B) Quantification of phagocytic (GFP⁺F4/80⁺) cell number in tumors (n = 5).

(C) Schematic diagram of the in vitro bead phagocytosis assay.

(D) Images of the in vitro BMDM bead phagocytosis assay. BMDMs were stained with CellVue Claret reagent (red); FITC-beads are shown in green. Arrowheads indicate phagocytic Mφs. Scale bar, 20 μm.

(E) Quantification of the phagocytic BMDM ratio. Three independent experiments were performed with two replicates.

(F) Images of the in vitro BMDM and LLC cell phagocytosis assay. BMDMs were stained with CellVue Claret reagent (red); LLC cells were stained with CFSE (green) and Hoechst (blue). Arrowheads indicate phagocytic Mφs. Scale bar, 20 μm.

(G) Quantification of the phagocytic BMDM ratio. Three independent experiments were performed.

(H) Schematic diagram of the ex vivo bead phagocytosis assay.

(I) Image of the ex vivo phagocytosis assay. Ly6C^lo Mφs were stained with CellVue Claret reagent (red); FITC-beads shown in green. Arrowheads indicate phagocytic Mφs. Scale bar, 20 μm.

(J) Quantification of the phagocytic Ly6C^lo Mφ ratio (n = 5).

Data represent means ± SEM. Statistical analysis was performed using the Mann–Whitney test. *p < 0.05; **p < 0.01. See also Figure S5.