The authors of “The eps8 Family of Proteins Links Growth Factor Stimulation to Actin Reorganization Generating Functional Redundancy in the Ras/Rac Pathway” (Mol. Biol. Cell [2004] 15, 91–98; originally published online as 10.1091/mbc.E03-06-0427) wish to make a correction to Figure 4B of the article.
Figure 4.
Interaction between actin and eps8 family members. (A) 293T cells were transfected with flag-tagged eps8 family members (as indicated at the top, Tfx), followed by immunoprecipitation (IP) with an antiflag (F) or control IgGs (c). Lysates and immunoprecipitates were immunoblotted (WB) with the indicated antibodies. The flag (bottom) panel is the same as in Figure 2, bottom, because the two experiments were performed together. (B) Actin cosedimentation assay. The indicated C-terminal fragments of eps8 and eps8 related proteins (eps8L1 [537–724]; Eps8L2 [551–716]; eps8L3 [509–594]; eps8 [586–821]) fused to GST (1 μM) were mixed in the presence (+ actin) or absence of 10 μM of purified F-actin, as described in MATERIALS AND METHODS, and subjected to ultracentrifugation. GST alone was used as a negative control. Each lane shows the supernatant (S) and the pellet (P) obtained after ultracentrifugation. The lanes (actin) on the left show the supernatant and the pellet of F-actin alone. Detection was by immunoblotting (WB) with the indicated abs. (C) MEFs, microinjected with flag-tagged eps8 family members (indicated on the left), were serum-starved and stimulated with 10 ng/ml PDGF (+PDGF) or mock-treated (–PDGF). Cells were fixed and stained with an antiflag antibody (Anti-Flag, green) or rhodaminated phalloidin to visualize F-actin (Phalloidin, red).
It has been brought to our attention that in the original HTML and PDF versions, during the assembly of Figure 4B, we made the following errors during the assembly of the final Figure 4B from the original autoradiograms and nitrocellulose membranes:
a) We erroneously flipped by 180° the top rightmost blot; and
b) we inadvertently duplicated the rightmost blot, and duplicated and erroneously trimmed the bottom leftmost.
We apologize for these errors and oversights. The corrected Figure 4 is shown below. These corrections do not change the interpretation of the published experiments.