(A, A’) Dac expression in Drosophila third instar larval eye-antennal imaginal disc. (A’) Split channel showing Dac expression alone in the eye imaginal disc. Note that arrow marks the MF in the eye imaginal disc. (B-E) In stalk-eyed fly, Dac expression in (B-D) eye-antennal imaginal disc, (E) pupal retina. (C, D) Magnified view of (C) eye and (D) antennal region. The stalk of the stalk-eyed fly eye imaginal disc is not visible due to folding. (E) In stalk-eyed fly, Dac is not expressed in the pupal retina, its expression is restricted to a band of cells in the optic lobe. Note that eye discs are stained for Dac (Green) and proneural marker Elav (Red). [AN: antenna, OL: optic lobe].