KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-human (H+L) Alexa 488 | Thermofisher | Cat# A-11013 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Human PD-L1 hIgG1 | R&D Systems | Cat# 156-B7–100 |
| Human PD-L2 hIgG1 | R&D Systems | Cat# 1224-PL-100 |
| Deposited Data | ||
| mouse PD-1 and PD-L2 complex | (Lazar-Molnar et al., 2008) | PDB: 3BP5 |
| human programmed death-1 (PD-1) and its ligand PD-L1 | (Zak et al., 2015) | PDB: 4ZQK |
| Experimental Models: Cell Lines | ||
| HEK 293-F Freestyle | Thermofisher | Cat# R79007 |
| Oligonucleotides | ||
| DNA primers | see Table S3 for all primers | |
| Recombinant DNA | ||
| human PD1 cDNA | GeneCopoeia | Cat# EX-B0169-M98 |
| mChery N1 | ClonTech | Cat# 632523 |
| Software and Algorithms | ||
| Gromacs | Gromacs v5.0.6 | http://www.gromacs.org |
| Pymol | Pymol v1.7 | https://sourceforge.net/projects/pymol/files/pymol/1.7/ |
| Modeller | (Sali and Blundell, 1993) | https://salilab.org/modeller/ |
| BeAtMusic | (Dehouck et al., 2013), | http://babylone.ulb.ac.be/beatmusic/ |
| FoldX | (Schymkowitz et al., 2005) | http://foldxsuite.crg.eu/ |
| MutaBind | (Li et al., 2016) | https://www.ncbi.nlm.nih.gov/research/mutabind/ |
| ProtLID | (Yap and Fiser, 2016). | N/A |
| matplotlib | Python version 2.7.10 | https://matplotlib.org |
| pandas | Python version 2.7.10 | https://pandas.pydata.org/index.html |
| numpy | Python version 2.7.10 | http://www.numpy.org/reference/stats.html |
| scipy | Python version 2.7.10 | https://docs.scipy.org/doc/scipy |