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Molecular screening for CALR mutation. Screening for CALR mutation was performed by fragment analysis at diagnosis of CML (panel A), and 3 months later when the thrombocytosis appeared (panel B). Digital PCR at the time of diagnosis of CML was able to detect the CALR type 1 mutation with a VAF of 0.8% (panel C). VAF = variant allele frequency, WT = wild-type allele.
