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. Author manuscript; available in PMC: 2019 Sep 16.
Published in final edited form as: Bone. 2017 Jan 21;97:153–167. doi: 10.1016/j.bone.2017.01.022

Figure 7.

Figure 7.

(A) An immunoblot analysis of a time course of ERK1/2 activation (p-ERK1/2) in RAW264.7 cells stimulated with RANKL in the presence of control-CM or hCTHRC1-CM is shown. The corresponding quantification of the signal marked with * is shown in (C). In (B) p-ERK1/2 levels in RAW264.7 cells growing in differentiation medium (DM) for up to 2 days are shown with corresponding quantification shown in (D). (E) hCTHRC1 inhibits RANKL-induced AP-1 luciferase reporter activity in RAW264.7 cells transfected with the AP-1 promoter reporter plasmid. (F) Western blot analysis of p-ERK1/2 level in femur lysates from wildtype and Cthrc1 null mice. All data represent means±SEM of replicates ≥3 with * = p<0.05.