TABLE 2.
Characterization of UbiV proteins and UbiU-UbiV heterodimeric complexesa
| Proteins | A280/A410 | Content (nmol/nmol of protein) |
|
|---|---|---|---|
| Iron | Sulfur | ||
| UbiV WT | 5.8 | 3.9 ± 0.13 | 3.3 ± 0.18 |
| UbiV C193A C197A | 8.4 | 2.4 ± 0.02 | 2.2 ± 0.13 |
| UbiV C39A C193A C197A | 15.5 | 1.9 ± 0.01 | 1.5 ± 0.4 |
| UbiV C180A C193A C197A | 26.7 | 0.7 ± 0.05 | 0.6 ± 0.1 |
| UbiV C39A C180A C193A C197A | 57.4 | 0.1 ± 0.04 | 0 |
| UbiU WT-UbiV WT | 4.3 | 7.9 ± 0.05 | 7.5 ± 0.06 |
| UbiU C169A C176A-UbiV WT | 7.3 | 4.7 ± 0.0.3 | 5.8 ± 0.1 |
| UbiU C193A C232A-UbiV WT | 8 | 3.9 ± 0.05 | 5.8 ± 0.1 |
a
Shown are metal content and UV-Vis properties after attempts of anaerobic reconstitution of their Fe-S centers for both wild-type and mutant proteins.