TABLE 3.
Oligonucleotides used for plasmid construction and mutant verification in this study
| Oligonucleotide | Sequence | Purpose |
|---|---|---|
| NdeI_pKK21-f | TATATCATATGCCCCGGCCCCGGCGCATG | Generation of pTE1624 |
| pKK21-r | TATATCCTAGGGCGGCGCCCCGCCTCCAGCGCGTC | |
| mCherry-f | TATATCCTAGGGCAGGGAGTGCGGCCGGCAG | |
| NdeI_mCherry-r | TATATCATATGTCACTTGTACAGCTCGTCCATG | |
| NdeI_pSYNiclXiclds-f | TATACATATGTCTAGAGGGCCGACAGGATTCGGCC | Generation of pTE1625 |
| HindIII_pSYNccrxccrds-r | TATATAAGCTTGCCGCTGCCGGCCGCACTCCCTGC | |
| HindIII_cerulean-f | TATATAAGCTTATGGTGAGCAAGGGCGAGGAGC | |
| NdeI_cerulean-r | TATATCATATGTTACTTGTACAGCTCGTCCATGCCGAG | |
| NdeI_icl-f | TATACATATGAGCAGAAAGACTTTTTCGGAAATC | Generation of pTE1614 |
| HindIII_icl-r | TATAAAGCTTCTATTCGGCGGCGAACTGGTTCATGGTG | |
| Pden_3873_ds-f | GGTCAGGCGCTTGTATTGGCCGAACATGTAG | Verification of TJE-KK5 |
| Pden_3873_ups-r | GCGAAAGCGGCATCGCCGTGGTGCGGATGAATTAC | |
| Pden_1363_ds-f2 | CATCCATTCATAGGCGGTGACCACCAGGCCC | Verification of TJE-KK6 |
| Pden_1363_ups-r | GCTGGGACTATATCTTCAGCTATATCAAGAC | |
| Pden_1363_ds-f2 | CATCCATTCATAGGCGGTGACCACCAGGCCC | Verification of TJE-KK10 |
| iclmCherryiclds_seq-f | CAGCATTGCCGAGGCCGACTACCCGGAC | |
| Pden_1363_ds-f | GACGAGCGCCGTGGTGAGTCTCAGCATGATGG | |
| Pden_1363_ups-r | GCTGGGACTATATCTTCAGCTATATCAAGAC | |
| NdeI mCherry-r | TATATCATATGTCACTTGTACAGCTCGTCCATG | Verification of TJE-KK13 |
| Pden_3873_ups-r | GCGAAAGCGGCATCGCCGTGGTGCGGATGAATTAC | |
| 3'-ccr-f | CGCAGGCGCATCTGAAGATGC | |
| Pden_3873_ds-f | GGTCAGGCGCTTGTATTGGCCGAACATGTAG | |
| Pden_1363_ds-f2 | CATCCATTCATAGGCGGTGACCACCAGGCCC | Verification of TJE-KK14 |
| Pden_1363_ups-r | GCTGGGACTATATCTTCAGCTATATCAAGAC | |
| HindIII_cerulean-f | TATATAAGCTTATGGTGAGCAAGGGCGAGGAGC | |