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. Author manuscript; available in PMC: 2020 Jun 5.
Published in final edited form as: Neuron. 2019 Apr 24;102(5):1037–1052.e7. doi: 10.1016/j.neuron.2019.03.037

Figure 1. PnocCeA Neurons Are Activated following Palatable, Calorically Dense Food Consumption.

Figure 1.

(A)Schematic of targeting of IRES-Cre cassette into the endogenous Prepronociceptin gene.

(B)Sagittal section of PnocIRES-Cre; Rosa26-flx-stop-fIx-TdTomato (AI9) mice.

(C–E) Example images of Pnoc (C; red), Cre (D; green), and merge (E) mRNA expression in the CeA. Scale bars represent 50 μM.

(F)Quantification of Pnoc, Cre, and double-positive cell counts across all CeA images.

(G)Schematic of tissue clearing, IDISCO+, light sheet microscopy, and ClearMap workflow in PnocIRES-Cre; AI9 mice.

(H)Quantification of Pnoc+ cells across the A-P axis following extraction of segmented Pnoc cell coordinates after ClearMap.

(I)Schematic of ex vivo recordings of Pnoc-GFP reporter mice.

(J) Average membrane capacitance (Cm) of Pnoc neurons.

(K) Average membrane resistance (Rm) of Pnoc neurons.

(L) Hyperpolarization-activated inward (Ih) currents in Pnoc neurons.

(M) Example low-threshold bursting in response to depolarizing current step.

For (J) and (K), n = 13 cells from 3 mice.