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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Neurotoxicology. 2019 Aug 2;74:221–229. doi: 10.1016/j.neuro.2019.07.008

Fig. 4. TCS effect on mRNA and protein expressions of apoptosis-related proteins in PC12 cells.

Fig. 4

The mRNA and protein expressions of apoptosis-related genes (including Bax and Bcl-2) were assessed in PC12 cells after treated with 1, 10, 50 μM TCS for 12 hr by using RT-qPCR and western blotting. Changes in mRNA expressions of Bcl-2 (A) and Bax (B) and Changes in protein expression of Bcl-2(C) and Bax (D) were measured. Band intensities were quantified with Image-Pro Plus 6.0. The protein expression of Bax and Bcl-2 were normalized to β-actin. Data were expressed as the fold of mRNA or protein expression of target genes relative to the Control. *p<0.05 or **p<0.01 vs. Control. N=5.