FIG 5.
SigN is a sigma factor that drives transcription in vitro. In vitro transcription assays using Pveg (left), PsigNUP (middle), and PsigNDN (right) promoter fragments in the presence (+) and absence (−) of a 5× molar ratio of SigN added to RNA polymerase holoenzyme purified from B. subtilis. The predicted transcriptional products resulting from PsigN1, PsigN2, and PsigN3 are indicated. Two products were observed from Pveg likely due to proper termination (short product) and terminator read-through (long product).