Therapeutic effect of transplantation of human bone marrow-derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion

Abstract

Human bone marrow-derived mesenchymal stromal cells (hBMSCs) have been revealed to be beneficial for the regeneration of tissues and cells in several diseases. The present study aimed to elucidate the mechanisms underlying the effect of hBMSC transplantation on neuron regeneration in a rat model of middle cerebral artery occlusion (MCAO). The hBMSCs were isolated, cultured and identified. A rat model of MCAO was induced via the modified Longa method. Neurological severity scores (NSS) were adopted for the evaluation of neuronal function in the model rats after cell transplantation. Next, the expression levels of nestin, β-III-tubulin (β-III-Tub), glial fibrillary acidic protein (GFAP), HNA and neuronal nuclear antigen (NeuN) were examined, as well as the positive expression rates of human neutrophil alloantigen (HNA), nestin, NeuN, β-III-Tub and GFAP. The NSS, as well as the mRNA and protein expression of nestin, decreased at the 1st, 2nd, 4 and 8th weeks, while the mRNA and protein expression of NeuN, β-III-Tub and GFAP increased with time. In addition, after treatment, the MCAO rats showed decreased NSS and mRNA and protein expression of nestin, but elevated mRNA and protein expression of NeuN, β-III-Tub and GFAP at the 2nd, 4 and 8th weeks, and decreased positive expression of HNA and nestin with enhanced expression of NeuN, β-III-Tub and GFAP. Therefore, the present findings demonstrated that hBMSC transplantation triggered the formation of nerve cells and enhanced neuronal function in a rat model of MCAO.

Introduction

Cerebral stroke is a leading cause of mortality and long-term disability among adults in developed countries; the majority (85%) are ischemic in nature and are caused by the closure of a major cerebral artery through an embolism or thrombus, which brings about subsequent tissue death and blood flow loss in the affected region (1). The cellular and molecular mechanisms that are responsible for the neurodegeneration induced by stroke involve acidosis, excitotoxicity, oxidative stress, bioenergetic failure and inflammation, leading to necrotic or apoptotic cell death (2). The neural network re-establishment of newborn neurons is regarded as the fundamental anatomic basis for the process of functional repair in injured brains, and many studies have been devoted to fully understanding the therapeutic mechanisms of rehabilitative treatment for patients with stroke (3,4). Advances in stem cell studies over the past 20 years have led to the potential use of stem cells in the treatment of degenerative diseases and neuronal injury (5–7). It has been reported that the nerve mobilization technique can improve nerve conduction and the axonal transport system and reduce intraneural compression within the nerves to enhance circulation to the nerves (8). Importantly, one study demonstrated that preconditioned transplanted bone marrow-derived mesenchymal stromal cells (BMSCs) could initiate the de novo regeneration of gastric neuronal cells and structures that, in turn, could restore gastric contractility; this result suggested that preconditioned allogeneic BMSCs exhibit therapeutic value for the treatment of enteric nerve disorders (9).

MSCs can be applied to hematopoietic stem cell transplantation, organ transplantation, and bone and cartilage tissue repair, along with treatment for myocardial infarction and liver injury (10,11). All the aforementioned functions are associated with the important characteristics of regeneration, but in different ways for different diseases. However, all these effects of MSCs are attributed to the regenerative power of stem cells in the lesion. The repair is believed to be induced through growth-stimulating factors provided by stem cells for tissue regeneration in the lesion (12). The supportive role of stem cells for various highly differentiated cells is responsible for these specific functions and lays the foundation for repair and regeneration in the lesion, as well as for replantation of suitable tissue (13,14). It has been elucidated that the recruitment of MSCs to the injury site is accomplished through chemotaxis, including via growth factors, which are of great significance to tissue regeneration from the perspective of cell survival, proliferation, migration and differentiation (15). Experts have also reported that the pro-angiogenic properties of human umbilical cord-derived MSCs in tissue growth and regeneration are related to structural support for capillary-like structures (16).

Adult BMSCs, namely multipotent progenitors, are capable of adipogenic, osteogenic and chondrogenic differentiation and can display transdifferentiation potential beyond the restrictions of the mesenchymal lineages, such as differentiation into neurons (17–20). Due to their high plasticity and active expansion capacity, as well as their typically low immunogenicity, BMSC therapy remains a unique and attractive strategy (21,22). A previous study demonstrated that following transplantation, BMSCs could develop into glial-type cells capable of improving peripheral nerve regeneration (23). Multiple animal models have been gradually developed as models for ischemic stroke, among which the middle cerebral artery occlusion (MCAO) model has been commonly adopted, especially in rodents (24). A recent study reported that MCAO stroke could cause the degeneration of nigral dopaminergic neurons in human brains, followed by ischemic neuronal death in the cerebral cortex, striatum and ischemic core (25). Studies have demonstrated that BMSC transplantation is able to enhance functional recovery after cerebral ischemia (26,27). Thus, the present study was conducted to explore the mechanisms underlying the effect of hBMSC transplantation on neuron regeneration in a rat model of MCAO. It was hypothesized that hBMSC transplantation could trigger the formation of nerve cells and enhance neuronal function in a rat model of MCAO.

Materials and methods

Study subjects

A total of 70 male Sprague-Dawley (SD) rats (10 weeks old; 250–280 g) were obtained from Hunan SJA Laboratory Animal Co., Ltd. All treatments on animals were in accordance with ‘On the Guidance to Take Care of the Experimental Animals’ issued by the Ministry of Science and Technology of China. Adult marrow was obtained from two surgical patients (female; 24 and 28 years old) and three orthopedic trauma patients (1 female, 34 years old; 2 males, 26 and 38 years old) in Renmin hospital of Wuhan University between September 2018 and December 2018. According to ‘Managerial Regulation of the Medical Institutions Administrative Rules’, promulgated by the State Council of China, the experimental procedures and risks were announced to the subjects, and informed consent was received from all subjects. All experimental animal operating procedures were in line with the US National Institutes of Health laboratory animal care and usage guidelines.

Isolation and culture of hBMSCs

In a sterile environment, 20 ml of healthy human marrow was extracted for the isolation of leukomonocytes, which were then used to obtain mononuclear cells through the density-gradient method (28). Next, the mononuclear cells were inoculated in DMEM/F12 (cat. no. 51985042; Gibco; Thermo Fisher Scientific, Inc.) containing 10% FBS and 1% glutamine at a concentration of 1×10⁹ l⁻¹, and cultured in a 5% CO₂ incubator with saturated humidity at 37°C. The solution was changed every 3 days. When the cell confluence reached 80%, the cells were detached using a mixed solution of 2.5 g/l trypsin and 0.2 g/l EDTA, and subcultured at a ratio of 1:2 for amplification.

Identification of hBMSCs

Cells at passage 5 were inoculated in a 24-well plate with a prepared sterile coverslip at a density of 4×10⁴/ml and cultured at 37°C under 5% CO₂. The cells were fixed with 4% paraformaldehyde at room temperature for 30 min, treated with 0.3% TritonX-100 at room temperature for 30 min, and blocked with goat serum (Thermo Fisher Scientific Inc.) for 1 h at room temperature. Next, the cells were incubated at room temperature for 3 h with the following primary antibodies: Rat anti-human CD34 (bsm-16538M, 1:100; BIOSS), CD44 (bsm-51065M, 1:100; BIOSS) and CD45 (bs-4819R, 1:100; BIOSS). After three washes with PBS with Tween-20 (PBST), the cells were incubated with FITC-labeled secondary goat anti-mouse antibody (bs-0293P, 1:100, BIOSS) at room temperature for 1 h, avoiding exposure to light. Finally, the cells were rinsed three times with PBST, mounted with neutral resin, and observed and photographed under a confocal laser scanning microscope (LSM 700; Carl Zeiss AG); the number of positive cells were counted in a random field per view.

MCAO model establishment and grouping

According to the modified Longa method (29), the rat model of MCAO was established. At 2 mm behind and 5 mm to the right of the fontanelle, laser Doppler flowmetry was used to monitor the blood flow in the brain before occlusion, after occlusion and after reperfusion. The ischemia was successfully induced when the blood flow after occlusion decreased to <30% of that before occlusion, and the reperfusion was shown to be successful when the blood flow increased to 70% of that before occlusion with no occurrence of basilar hemorrhage (29). During the surgery, an animal pulse oximeter was used to monitor the arterial oxygen saturation, breathing rate and heart rate of all rats. After the surgery, water and food were offered to the rats. According to the random number table method, the established rat models were randomly divided into treatment (injected with hBMSCs; n=35) and control (injected with PBS; n=35) groups. All rats received 8.0 Gy γ-radiation in the whole body to suppress immunity, and the transplantation was conducted on the 3rd day after radiotherapy. Rats in the treatment group were stereotactically injected with 0.5 ml of hBMSCs (1×10⁷ cells/rat) through the cerebral cortex, and rats in the control group were stereotactically injected with the same amount of PBS through the cerebral cortex. The Neurological Severity Score (NSS) was determined at 1, 2, 4 and 8 weeks after transplantation. Of note, 5 and 8 rats succumbed in the treatment and control groups, respectively. Thus, 6 surviving rats in each group were anesthetized and sacrificed by cervical vertebra dislocation to obtain whole brain tissues; of these tissues, one part was used for reverse transcription-quantitative PCR (RT-qPCR) and the other part was used for western blot analysis. The brain tissues at 1 week after transplantation were used for triphenyl-2,3,5-tetrazoliumchloride (TTC) staining, and the brain tissues at 8 weeks after transplantation were used for immunohistochemistry.

Stereotaxic injection

Rats were placed in the middle of the stereotaxic apparatus. The front teeth of the rats were locked on the anterior clamp. The clamp was lowered and tightened. The ear bars were inserted into the ear canal to stabilize the head; the insertion distance was limited to avoid any damage to the inner ear. The surgical site was sterilized by 70% ethanol and 10% iodophor using a cotton swab (two times). An incision was created at 1.5 cm above the ears (toward the nose) and extended to 0.5 cm below the ears. The incision was further extended vertically to the middle of the head to expose the bregma, followed by slow removal of the membrane on the surface of the skull using a sterilized cotton swab. The syringe (1 µl) was filled with cells with no bubbles and then placed in the intersection of the bregma and interaural line where the point was set at zero, with the coordinates determined using brain atlases. Then, the syringe was slightly raised to place the drill directly above the target coordinate at a ~45° angle to the skull. The reduction of resistance signaled the initiation of the next step. It is necessary that drill not be put into the brain in order to prevent cortical damage. The syringe was put at a depth of <2 mm and the injection started at a speed of 0.2 µl/min. It is necessary to ensure that the syringe does not slip lower than the desired depth. If this occurs, the syringe should be gently lifted back to the desired depth. Then, 2 min after the injection was completed, any residual cells were absorbed. The syringe was slowly lifted for injection into the other hemisphere. The scalp was sutured and sterilized with iodophor (30).

NSS

The NSS system was used for the evaluation of functional nerve damage and recovery in rats in the treatment and control groups at 1, 2, 4 and 8 weeks after surgery (31). The scores were as follows: 13–18 points was regarded as severe neurological impairment; 7–12 points as moderate nerve function damage; and 1–6 points as light nerve function damage. The NSS evaluates the motor function, beam test, sensory function, reflex activity and other responses that can comprehensively reflect nerve function. Higher scores indicate more severe symptoms, and lower scores indicate lighter symptoms (Table I).

Table I.

Detailed standards for neurological severity score points.

Items	Contents	Points
Electric shock experiments	Flexion of forelimb	1
	Flexion of hindlimb	1
	Head moved >10° to vertical axis within 30 sec	1
Motor tests	Normal walk	0
	Inability to walk straight	1
	Circling toward paretic side	2
	Falling down to paretic side	3
Sensory tests	Placing test (visual and tactile test)	1
	Proprioceptive test (deep sensation, pushing paw against table edge to stimulate limb 1 muscles)	1
Beam balance tests	Steady posture	0
	Grasps side of beam	1
	Hugging beam and 1 limb falls down from beam	2
	Hugging beam and 2 limbs fall down from beam, or spins on beam (60 sec)	3
	Trying to balance on beam but falling off (40 sec)	4
	Trying to balance on beam but falling off (20 sec)	5
	Falling off without attempt to balance or hang on to beam (20 sec)	6
Reflex absence and abnormal movements	Pinna reflex (head shaking when auditory meatus is touched)	1
	Corneal reflex (eyes blinking when cornea is lightly touched with cotton)	1
	Startle reflex (motor response to a brief noise from snapping a clipboard paper)	1
	Seizures, myoclonus, myodystony	1
Maximum points		18

TTC staining

At 1 week after transplantation, the rats were anesthetized with sodium pentobarbital (50 mg/kg; i.p.), and the rat brains were removed carefully. Next, the brains were cut into 1-mm coronal sections using a vibratome. The brain sections were immersed in 2% TTC solution with normal saline at 37°C for 30 min (32).

RT-qPCR

The whole brain tissue samples were removed at 1, 2, 4 and 8 weeks after transplantation and NSS determination. The total RNA was extracted using TRIzol™ (cat. no. 15596-018; Invitrogen; Thermo Fisher Scientific, Inc.), and the absorbance (A)260/A280 and RNA concentration were detected using a NanoDrop ultraviolet spectrophotometer (NanoDrop 2000; Thermo Fisher Scientific, Inc.). Five µg of cDNA was reverse transcribed according to the manufacturer's instructions of the cDNA kit (cat. no. K1622; Fermentas Inc., Ontario, CA, USA). The cDNA was synthesized via RT, according to the instructions of the Applied Biosystems StepOne™ (cat. no. 4376357, Thermo Fisher Scientific, Inc.) and StepOnePlus™ Real-Time PCR Systems (cat. no. 4376600, Thermo Fisher Scientific, Inc., Waltham, MA, USA) at room temperature. The PCR (cat. no. A25780, SYBR Green I, Thermo Fisher Scientific, Inc.) was conducted using the ABI 7500 RT-qPCR instrument (cat. no. AM1005; Invitrogen; Thermo Fisher Scientific, Inc.). The reaction conditions for nestin, β-III-tubulin (β-III-Tub), glial fibrillary acidic protein (GFAP) and neuronal nuclear antigen (NeuN) were as follows: Pre-denaturation at 95°C for 3 min, a total of 38 cycles of denaturation 95°C for 30 sec, annealing at 60°C for 40 sec and extension at 60°C for 40 sec. The fold changes between the experimental group and the control group were calculated by means of the 2^−ΔΔCq method for relative quantification. Each sample had three duplicates and all primers used above were synthesized by Sangon Biotech Co., Ltd. (Table II).

Table II.

Primer sequences for the reverse transcription-quantitative PCR.

Gene	Primer sequence (5′-3′)
Nestin	F: CAGCTGGCGCACCTCAAGATG
	R: AGGGAAGTTGGGCTCAGGACTGG
β-III-Tub	F: AGATGTACGAAGACGACGAGGAG
	R: GTATCCCCGAAAATATAAACACAAA
GFAP	F: GTGGGCAGGTGGGAGCTTGATTCT
	R: CTGGGGCGG CCTGGTATGACA
NeuN	F: GGGTTTTGGGTTTGTAACTTTTGAA
	R: AGACTGCTCCTACCACAGGGTTTAG
GAPDH	F: TCCCCACTGCCAACGTGTCAGTG
	R: ACCCTGTTGCTGTAGCCAAATTCG

β-III-Tub, β-III-tubulin; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclear antigen; R, reverse, F, forward.

Western blot analysis

The whole brain tissue samples were removed 1, 2, 4 and 8 weeks after transplantation and NSS. Next, 80 mg/kg samples were added to 1 ml of tissue lysate (cat. no. 89900, Thermo Fisher Scientific, Inc), 40 mg (~5×10⁶ cells) of wet cell precipitation, ground into homogenate in an ice bath, and lysed to obtain protein lysate at 4°C for 30 min with shaking every 10 min. Next, the samples were centrifuged at 25,764 × g at 4°C for 20 min to eliminate the lipid layer. The liquid supernatant was obtained, and a bicinchoninic acid kit (cat. no. 20201ES76; Yeasen, Inc.) was adopted to detect the protein concentration of each sample. Deionized water was used to adjust the loading sample to 30 µg protein/lane. Next, the proteins were separated by 10% SDS-PAGE, transferred onto a nitrocellulose membrane and blocked with 5% skimmed milk powder at 4°C overnight. Next, the membrane was incubated with rabbit anti-β-III-Tub (cat. no. ab18207; 1:1,000), GFAP (cat. no. ab7260; 1:5,000), nestin (cat. no. ab221660; 1:1,000) and NeuN (cat. no. ab177487; 1:2,000) in a sealed plastic bag at 4°C overnight, followed by three PBST washes (5 min each) at room temperature. Next, the membrane was incubated with horseradish peroxidase (HRP)-labeled goat anti-rabbit secondary antibody immunoglobulin G (IgG; cat. no. ab6721; 1:5,000) at 37°C for 1 h and washed three times with PBST (5 min each) at room temperature. The membrane was soaked in ECL solution (cat. no. ECL808-25; Biomiga, Inc.), subjected to the reaction at the room temperature for 1 min with the liquid eliminated, and then developed in the dark. GAPDH (1:10,000, ab181602, Abcam) was used as the internal reference, and the relative protein expression was calculated via the ratio of the gray value of the target band to that of the internal reference band (Quantity One, v4.6.6, Bio-Rad Laboratories, Inc.).

Immunohistochemistry

A total of 8 weeks after transplantation, the rats were anesthetized and subjected to reperfusion with normal saline and 40 g/l paraformaldehyde through the heart. Cervical vertebra dislocation was performed to obtain the brain tissues. The brain tissues were placed in normal saline at 1–4°C, embedded in optimal cutting temperature compound and cut into 5-µm frozen sections. Next, the sections were treated with 3% H₂O₂ for 30 min to block endogenous peroxidase activity, incubated in 0.3% Triton X-100/0.1% BSA (Thermo Fisher Scientific, Inc.) at 37°C for 2 h, and incubated in a wet box at 4°C overnight with rabbit antiβ-III-Tub polyclonal antibody (cat. no. ab18207; 1:200), GFAP (cat. no. ab7260; 1:100), nestin (cat. no. ab92391; 1:250), NeuN (cat. no. ab104224; 1:5,000), HNA (cat. no. ab215396; 1:100) and GAPDH (cat. no. ab8245; 1:1,000). PBS at a concentration of 0.01 mol/l was adopted as the NC. Following the incubation, the sections were washed three times with PBST, incubated with HRP-labeled rabbit anti-mouse secondary antibody IgG (cat. no. ab6725; 1:1,000) or goat anti-rabbit secondary antibody IgG (cat. no. ab6721; 1:1,000) at 37°C for 1 h, and washed three times with PBST. Lastly, the sections were stained with diaminobenzidine at room temperature for 10 min, and brown staining was verified as a positive reaction. A total of five visual fields were randomly selected in each section for photographing under an inverted microscope (XDS-800D; magnification, ×200; Canon, Inc.); the positive expression rate was calculated.

Statistical analysis

SPSS 21.0 (IBM Corp.) was used for the data analysis. The measurement data are presented as the mean ± standard deviation. The experiment was repeated 3 times. An independent sample t-test was used for comparisons between two groups. The enumeration data are shown as the typical value of the cases. The χ² test was applied for comparisons between two groups. P<0.05 was considered to indicate a statistically significant difference.

Results

Identification of hBMSCs

The immunofluorescence assay results showed that no positive expression of CD34 and CD45 appeared in the hBMSCs after isolation and culture, suggesting that the isolated cells were not hemopoietic stem cells, since both the surface molecules CD34 and CD45 should be positive if so. The positive expression rate of CD44 was 92.75%, indicating that the majority of the isolated cells were hBMSCs, due to the fact that CD44 serves as the main surface marker for cell identity concerning hBMSCs (33). These findings demonstrated that the cultured hBMSCs in the present study were suitable for use in further experiments (Fig. 1).

Figure 1.

Expression of BMSC surface markers (CD34, CD44 and CD45) detected by the immunofluorescence assay and the positive rate of CD44 is 92.75%, indicating that most of the cells we isolated are human bone marrow mesenchymal stem cells, conforming to further experimental requirements. (A) CD34, CD44 and CD45, as examined by immunofluorescence assay (magnification, ×400). Green, surface markers; blue, nuclei. (B) Positive expression rates of CD34, CD44 and CD45.

Successful establishment of the MCAO rat model

For all 70 rats in the treatment and control groups, after ligation of the middle cerebral artery by strings with diameters of 0.36 and 0.38 mm, the cerebral blood flow of the 70 mice was reduced to <30% of that before occlusion and, after reperfusion, increased to >70% of that before occlusion. The following symptoms occurred 24 h after anesthesia: Lack of contraction of the left limb upon pain stimulation, occurrence of circling or dumping and the inability to extend straight forward upon lifting the tail. This observation indicated that the model establishment rate was 100%. In total, five rats succumbed in the treatment group, with a survival rate of 85.7%, and eight rats succumbed in the control group, with a survival rate of 77.1%, three died in the first week, six died in the second week, and four died in the third week.

NSS decreases remarkably in MCAO rats after hBMSC transplantation

NSS was applied to evaluate the nerve function damage and recovery condition of rats in the treatment and control groups at 1, 2, 4 and 8 weeks after surgery. Scores of 13–18 points were regarded as severe neurological impairment, 7–12 points as moderate nerve function damage, and 1–6 points as light nerve function damage. The NSS evaluates motor function, the beam test, sensory function, reflex activity and other indicators, which comprehensively reflect nerve function. The higher the score, the more serious the symptoms. The results of NSS evaluation revealed that the rats mainly showed moderate nerve function damage 1 week after surgery, and this damage decreased with time. The NSS presented a generally decreasing trend both in the treatment and control groups 1, 2, 4 and 8 weeks after surgery. Compared with the control group, the NSS decreased significantly in the treatment group 2, 4 and 8 weeks after surgery (P<0.05; Fig. 2).

Figure 2.

NSS significantly decreases in MCAO rats after hBMSC transplantation. *P<0.05 vs. control group. NSS, neurological severity score; hBMSCs, human bone-marrow-derived mesenchymal stem cells; MCAO, middle cerebral artery occlusion.

Infarct areas are reduced in MCAO rats after hBMSC transplantation

TTC staining was conducted using the brain tissues of MCAO rats to observe the severity of obstruction in the rats according to the infarct areas. The results of the TTC staining revealed that rats in the control group showed larger infarct areas than those in the treatment group (P<0.05; Fig. 3). Therefore, MCAO rats showed reduced infarct areas after hBMSC transplantation.

Figure 3.

Decreased infarct areas are found in MCAO rats after hBMSC transplantation. (A) The brain tissues of rats in the control and treatment groups, as examined by triphenyl-2,3,5-tetrazoliumchloride staining. (B) The infarct volumes of rats in the control and treatment groups. *P<0.05 vs. control group. MCAO, middle cerebral artery occlusion; hBMSCs, human bone marrow-derived mesenchymal stem cells.

mRNA expression of neuronal biomarkers increases after hBMSC transplantation in MCAO rats

RT-qPCR was conducted to examine the mRNA expression of brain tissue neuronal biomarkers. The results showed that the mRNA expression of nestin decreased in the control and treatment groups with time at 1, 2, 4 and 8 weeks after hBMSC transplantation, and it was lower in the treatment group than in the control group (P<0.05; Fig. 4A). The mRNA expression of NeuN, β-III-Tub and GFAP was elevated significantly in both groups and was higher in the treatment group than in the control group (all P<0.05; Fig. 4B-D).

Figure 4.

mRNA expression of nestin, NeuN, β-III-Tub and GFAP increases after hBMSC transplantation. (A) mRNA expression of nestin at 1, 2, 4 and 8 weeks after hBMSC transplantation. (B) mRNA expression of NeuN at 1, 2, 4 and 8 weeks after hBMSC transplantation. (C) mRNA expression of β-III-Tub at 1, 2, 4 and 8 weeks after hBMSC transplantation. (D) mRNA expression of GFAP at 1, 2, 4 and 8 weeks after hBMSC transplantation. *P<0.05 vs. respective control group. β-III-Tub, β-III-tubulin; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclear antigen; hBMSCs, human bone marrow-derived mesenchymal stem cells.

Protein expression of nestin, NeuN, β-III-Tub and GFAP increases after hBMSC transplantation

Western blot analysis was applied to examine the protein expression of nestin, NeuN, β-III-Tub and GFAP 1, 2, 4 and 8 weeks after hBMSC transplantation, and the results showed that the protein expression of nestin in both groups decreased with time and was significantly decreased in the treatment group compared with the control group (P<0.05). The protein expression of NeuN, β-III-Tub and GFAP was elevated in both groups, and increased significantly in the treatment group compared with the control group (P<0.05; Fig. 5).

Figure 5.

Protein expression of nestin, NeuN, β-III-Tub and GFAP increases after hBMSC transplantation. (A) Protein bands for nestin, NeuN, β-III-Tub, GFAP and GAPDH 1 week after hBMSC transplantation. (B) Protein bands for nestin, NeuN, β-III-Tub, GFAP and GAPDH 2 weeks after hBMSC transplantation. (C) Protein bands for nestin, NeuN, β-III-Tub, GFAP and GAPDH 4 weeks after hBMSC transplantation. (D) Protein bands for nestin, NeuN, β-III-Tub, GFAP and GAPDH 8 weeks after hBMSC transplantation. *P<0.05 vs. respective control group. β-III-Tub, β-III-tubulin; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclear antigen; hBMSCs, human bone marrow-derived mesenchymal stem cells.

Positive expression rates of NeuN, β-III-Tub and GFAP are increased after hBMSC transplantation

At 8 weeks after hBMSC transplantation, immunohistochemical staining showed that the positive expression rates of HNA, nestin, NeuN, β-III-Tub and GFAP after hBMSC transplantation in the treatment group were 14.8, 11.8, 78.0, 74.2 and 36.2%, respectively, and those in the control group were 72.2, 65.8, 22.2, 20.2 and 9.4%, respectively. These results suggested that the positive expression rates of HNA and nestin decreased significantly, while those of NeuN, β-III-Tub and GFAP after hBMSC transplantation in the treatment group were elevated significantly compared with those in the control group (all P<0.05; Fig. 6).

Figure 6.

Differentiation of hBMSCs into neural cells in the rat brain after transplantation. (A) HNA, nestin, NeuN, β-III-Tub and GFAP in the control and treatment groups, as examined by immunohistochemistry. Cell staining after hBMSC transplantation in the case and control groups is presented. (B) Cell numbers for HNA, nestin, NeuN, β-III-Tub and GFAP in the treatment and control groups. *P<0.05 vs. control group. β-III-Tub, β-III-tubulin; GFAP, glial fibrillary acidic protein; HNA, human neutrophil alloantigen; NeuN, neuronal nuclear antigen; hBMSCs, human bone marrow-derived mesenchymal stem cell.

Discussion

Stroke is a major cause of mortality worldwide, and it remains a significant cause of long-term disability among adults; ~87% of stroke cases are ischemic in origin and occur in the territory of the middle cerebral artery (34). It has been demonstrated that BMSC transplantation can improve functional recovery after cerebral ischemia (35,36). It has been reported that the nerve mobilization technique can improve nerve conduction and the axonal transport system, and reduce intraneural compression within the nerves, thus improving circulation to the nerves (8). Therefore, the present study aimed to explore the mechanisms underlying the effect of hBMSC transplantation on neuron regeneration in a rat model of MCAO, to develop a therapeutic method for enhancing nerve function following stroke. Compared with the control group, the case group exhibited significantly decreased NSS in the 2nd, 4 and 8th weeks compared with the control group. The modified NSS test has been commonly adopted to evaluate neurological function (37). One study tested the hypothesis that upon transplantation into the ischemic boundary zone, hBMSCs could differentiate, survive and improve functional recovery after MCAO (38). Neurological disorders may reduce the range of motion of the knee joint, which can affect the physiological movement of the spine and the pelvis, triggering excessive pelvic inclination during walking (39). Adult neurogenesis often consists of one or more of the following processes: Survival, proliferation, migration and differentiation (40). Additionally, it is well-known that neurogenesis is closely associated with the cell cycle, and neural stem cells may play roles by exiting the cell cycle (41). In addition, modification of the molecular signals and morphogens in the microenvironment of the developing brain influences stem cell survival and differentiation (42). Neurons have been found to be generated successfully from stem cells after culture, which provides a method to develop a stem cell-based transplantation strategy for human patients (43). Transplantation of hBMSCs reduces stereotypical behaviors and cognitive rigidity, and improves social behavior (44). In the present study, the NSS decreased significantly after hBMSC transplantation, indicating that hBMSC transplantation could promote nerve cell regeneration and enhance the neuronal function of human patients, in line with the aforementioned previous studies.

Compared with the control group, in the case group, the mRNA and protein expression and positive expression rates of nestin were significantly decreased, while those for NeuN, β-III-Tub and GFAP were elevated. Nestin, a class VI intermediate filament protein, was originally regarded as a neuronal stem cell biomarker in the development of the central nervous system, and it has been widely used in this capacity (45). Nestin has often been observed in the anterior, intermediate and neural lobes among scattered cells (46). GFAP is commonly regarded as a specific predictor of neurological outcome and brain damage in patients with traumatic brain injury (47). NeuN, a nuclear protein, is commonly expressed in mature post-mitotic neurons, and altered NeuN expression is a useful marker for immature or injured neurons (48). It is often localized in the perinuclear cytoplasm and the nuclei of neurons in the central nervous system (49). One study reported that BMSCs contain a highly mobile number of C-X-C chemokine receptor type 4-A-positive cells that express mRNA for different biomarkers of early tissue-committed stem cells, including β-III-Tub, nestin, NeuN and GFAP (50). Another study demonstrated that co-culture of amyloid-β treated neuronal progenitor cells with BMSCs markedly increases the expression of Ki-67, GFAP and ELAV like RNA binding protein 4, indicating that BMSCs can modulate endogenous adult neurogenesis for Alzheimer's disease treatment (51). Thus, it was concluded that hBMSC transplantation may promote nerve cell regeneration and the neuronal function of human patients. All of these effects of MSCs described above are attributed to the ability of stem cells to regenerate in lesions and the determinants of growth factors and structurally supported release (52). Repair is thought to be induced by growth stimulators provided by stem cells for tissue regeneration in injury. Importantly, the regenerative effects of MSCs under injury conditions are closely associated with the release of cell protection and survival-related factors (53,54). The support of stem cells for various highly differentiated cells is the cause of this specific function, laying the foundation for the repair and regeneration of lesions and the replantation of appropriate tissues.

MSCs derived from adult tissues present an potentially important progenitor cell source for applications in tissue engineering and regenerative medicine. Modalities may include direct implantation and/or ex vivo tissue engineering, in combination with biocompatible/biomimetic biomaterials and/or natural or recombinantly-derived biologics. MSCs may also be considered for gene therapy applications for the delivery of genes or gene products (13,14). Another prospect for the future is the use of MSCs to create ‘off-the-shelf’ tissue banks. Karow et al (55) demonstrated that reprogramming of somatic cells into neurons offers a novel avenue for cell-based therapy for neurodegenerative diseases; a major challenge for this process is that it is uncertain whether the adult brain contains a population of cells that can be transformed directly into somatic cells. Crisan et al (56) reported that the vascular wall contains a number of progenitor cells that may be associated with the origin of elusive MSCs and other related adult stem cells. To fully harness the potential of these cells, future studies should be directed towards ascertaining their cellular and molecular characteristics for optimal identification, isolation and expansion, and to understand the natural, endogenous role(s) of MSCs in normal and abnormal tissue functions.

In conclusion, the present study revealed that hBMSC transplantation improved neuron regeneration in a rat model of MCAO, providing a possible therapeutic strategy for enhancing the nerve function of patients following stroke. However, due to the limited data and experimental environment, many improvements remain to be made in transplantation technology and experimental methods. It should also be noted that the study results may be affected by the limited sample size, and they should be further verified and observed in larger sample sizes. Additionally, the present study focused on exploring the function of MSCs without paying much attention to the regenerative power of MSCs, which might be studied in future research. In the future, research will be performed to uncover the typical mechanisms underlying the effect of hBMSC transplantation on neuron regeneration to provide a better and more effective treatment for neuronal dysfunction in patients after MCAO.

Funding

The present study was supported by the Fundamental Research Funds for the Central Universities (grant no. 2042017kf0139), Innovative Seed Funds of Medical College of Wuhan University (grant no. TFZZ2018027), Natural Science Foundation of Hubei Province (grant no. ZRMS2019000278) and the National Natural Science Foundation of China (grant no. 81301052).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

PX, MD, QGS and YGM wrote the paper, and conceived and designed the experiments. YZ and JHM analyzed the data. QC and SQL collected and provided the samples for the study. JQL, JC and FW obtained the results and conducted data analysis. JQL, JC and FW contributed to drafting the manuscript. All authors participated in the revised manuscript, and have read and approved the final submitted manuscript.

Ethics approval and consent to participate

The study was approved by the institutional review board of Renmin Hospital of Wuhan University. The experimental procedures and risks were announced to the subjects, and written informed consent was obtained from all participant. All animal experimentation in this study was approved by the Institutional Animal Care and Use Committee of Renmin Hospital of Wuhan University.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.