Fig. 1.

High-throughput microRNA inhibitor screening identified microRNAs that modulate androgen receptor signalling in prostate cancer (PC) cells. a Schematic illustration of AR reporter construct and derivative cell lines used for miR inhibitor screening. b Venn diagram illustrating common hits between LNCaP and C42 cells. c Correlation of miR hit scores between LNCaP and C42 cells. d–f Luciferase assay analysis of AR activity in LNCaP (i, ii) and C42 (iii, iv) cells transfected with 0–20 nM miR-346 (d), miR-361-3p (e) or miR-197-3p (f) inhibitor (i, iii) or mimic (ii, iv) for 24 h. Mean of three independent experiments ± SEM is shown. *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0001. See also Supplementary Table 2