Fig. 2.
STAT5B controls initial transformation and cell proliferation. a BM cells of wt, Stat5a−/−, or Stat5b−/− mice were infected with a retrovirus encoding BCR/ABLp185 and plated in growth factor-free methylcellulose. Representative pictures of colony formation assays (CFA) are depicted. Bar graph summarizes data obtained from 4 cell lines/genotype each in technical duplicates. Error bars represent mean ± SEM. Levels of significance were calculated using Kruskal–Wallis test followed by Dunn’s test. b Protein levels of BCR/ABL in wt, Stat5a−/−, or Stat5b−/− cell lines as determined by immunoblotting. One representative blot is shown. β-Actin served as a loading control. c Cell cycle profiles analyzed by PI-staining of BCR/ABLp185-transduced cell lines (n = 2 cell lines/genotype). Error bars represent mean ± SEM. Right: Growth curves of established wt, Stat5a−/−, or Stat5b−/− BCR/ABLp185+ cell lines (n = 2 cell lines/genotype). Error bars represent mean ± SEM. Levels of significance were calculated using Kruskal–Wallis test followed by Dunn’s test. STAT signal transducers and activators of transcription, BM bone marrow, wt wild type, PI propidium iodide