Fig. 4.

STAT5B suppresses interferon responses in BCR/ABL⁺ cells. RNA-seq analysis was performed on BCR/ABL^p185+ cell lines derived from wt, Stat5a^−/− and Stat5b^−/− mice. a Significantly (NES >1, FDR <0.25) enriched hallmark gene sets obtained from GSEA of log 2 fold-change ranked gene lists from differential expression analysis of Stat5b^−/− (vs. wt) and Stat5a^−/− (vs. wt) BCR/ABL^p185+ cell lines. Gene sets more enriched in Stat5b^−/− (left panel), only enriched in Stat5b^−/− (middle panel), and equally enriched in Stat5b^−/− and Stat5a^−/− (right panel) are shown. b Enrichment plots of the hallmark IFN-α response gene set from GSEA above and additional GSEA of differentially regulated genes between Stat5b^−/− and Stat5a^−/− BCR/ABL^p185+ cell lines. c The change in expression levels of genes that contribute to core enrichment of the hallmark IFN-α response gene set in Stat5b^−/− (orange) and Stat5a^−/− (blue) BCR/ABL^p185+ cell lines relative to wt BCR/ABL^p185+ cell lines and the significance of this change (p-adjust, see legend) is shown. IFN interferon, STAT signal transducers and activators of transcription, wt wild type, NES normalized enrichment score, GSEA gene set enrichment analysis, p-adjust Benjamin–Hochbert corrected p value, FDR false discovery rate