Fig. 3.

PRMT1 influences IGF-1 signaling. a MCF-7 cells were transfected with si:scramble or a pool of siRNAs targeting PRMT1 for 72 h, and then treated with IGF-1 for different times. Cell lysates were subsequently coimmunoprecipitated with the anti-IGF-1R antibody and detected by western blot analysis for the presence of ERα and IGF-1R, using the corresponding antibodies. The expression of mERα, ERα, PRMT1 and proteins involved in IGF-1 signaling was also evaluated by western blot using the corresponding antibodies. GAPDH expression was also assessed as a loading control. b MCF-7 cells were treated with the PRMT1 inhibitor (60 nM) 48 h before IGF-1 treatment, cell lysates were immunoprecipitated with anti-IGF-1R antibody and detected by western blot for the presence of IGF-1R and ERα, downstream IGF-1 signaling was then studied by western blot using the corresponding antibodies as in Fig. 3a. GAPDH expression was also assessed as a loading control. IGF-1 R insulin-like growth factor 1 receptor