Fig. 6.

Effect of VPA either alone or combined with IM in a GIST430 xenograft animal model. a–d GIST430 xenografts were established as described in the Materials and Methods section. Mice received an intraperitoneal injection of control (DMSO), 100 mg/kg IM, 200 mg/kg VPA, 100 mg/kg IM with 200 mg/kg VPA, and 500 mg/kg VPA, were administered i.p. twice per week for 4 weeks (n = 8/group). a Tumor volume was calculated as 1/2 x length x width². b The tumors were collected at the end of drug administration and analyzed by immunostaining (c) against KIT⁷⁰³, RELA, or KIT. The immunoexpression levels were quantified (d). The data are expressed as the means ± SD of three or more independent experiments. *p < 0.001