Table 1.

Primary and putative secondary driver mutations in young and old PV patients

Young PV sample	chr	start	ref	var	type	gene	strand	mut type	aa change	Tumor VAF
331060	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	16.5
465783	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	18.93
551599	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	45.51
638517	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	39.9
697576	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	13.6
732651	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	17.36
993645	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	11.03
Old PV sample	chr	start	ref	var	type	gene	strand	mut type	aa change	Tumor VAF
149502	20	31022264	G	A	SNP	ASXL1	1	nonsense	p.W583*	11.13
149502	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	5.14
208617	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	46.87
336362	20	31021250	C	T	SNP	ASXL1	1	nonsense	p.R417*	50
336362	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	33.25
374024	4	106196695	-	A	INS	TET2	1	frame_shift_ins	p.T1676fs	41.8
374024	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	39.62
374024	20	31022441	-	G	INS	ASXL1	1	frame_shift_ins	p.G645fs	39.78
513509	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	17.52
513509	22	29105992	A	C	SNP	CHEK2	−1	splice_site	e7+2	16.57
513884	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	17.91
513884	20	31023561	GCTGACA	-	DEL	ASXL1	1	frame_shift_del	p.A1016fs	6.71
570655	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	25.37
570655	2	25464537	C	T	SNP	DNMT3A	−1	missense	p.R659H	7.87
673286	9	5073770	G	T	SNP	JAK2	1	missense	p.V617F	92.24
673286	3	71027072	G	A	SNP	FOXP1	−1	nonsense	p.Q419*	50
673286	12	54686735	T	-	DEL	NFE2	−1	frame_shift_del	p.Y182fs	44.33

chr = chromosome; ref = reference; var = variant; mut type = mutation type; aa = amino acid; VAF = variant allele frequency; INS = insertion; DEL= deletion; SNP = single nucleotide polymorphism