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. 2019 Sep 24;10(5):e01743-19. doi: 10.1128/mBio.01743-19

FIG 4.

FIG 4

CyaA blocks upregulation of phagocytic capacity. CD14+ monocytes were cultured for 5 days in DMEM–20 ng/ml M-CSF and 22.5 pM CyaA or CyaA-AC− or the indicated compounds at the given concentrations. (A and B) Exposure to 4 ng/ml (22.5 pM) CyaA (A) or infection with the B. pertussis WT strain (B. pertussis AC+ PT+) (B) for 12 h at an MOI of 2:1 suppressed the differentiation-induced increase in the ability to phagocytose serum-opsonized 0.75-μm-diameter fluorescent phagobeads. n ≥ 4; **, P < 0.05; ***, P < 0.005. (C to E) CyaA (22.5 pM)-elicited cAMP signaling inhibited expression of phagocytic receptors FcγRI (C) and FcγRIII (D), and this inhibition was relieved by the activity of PKA-selective inhibitor Rp-8-Br-cAMPS (0.5 mM) (E). n ≥ 3 biological replicates; *, P < 0.05; **, P < 0.005; ***, P < 0.0005.