Skip to main content
. 2019 Jul 22;160(10):2271–2281. doi: 10.1210/en.2019-00297

Figure 5.

Figure 5.

Optogenetic stimulation of GH pulses in vivo. (A) Colabeling of dtTomato and GH in the pituitary from a GH-Cre mouse injected with Cre-activated AAV5-GAG-ChR2-dtTomato particles. (B) Laser light illumination of the ventral pituitary side in terminally anesthetized mice subjected to tail-tip blood sampling (3 µL every 3 min). (C) In experimental conditions as in (B), trains of blue laser light pulses (300-ms pulses at 1 Hz) triggered GH pulses (n = 5 male mice). (D) Laser light illumination of the pituitary in the awake mouse in which tail-tip blood sampling was carried out. (E) GH pulses triggered by a train of laser light pulses (300-ms pulses at 1 Hz) in GH-Cre mice injected with Cre-selective AAV5-GAG-ChR2-dtTomato particles (n = 5 male mice). Data can be viewed in materials saved to an online repository (13).