Skip to main content
PMC home page
Journal of Clinical Laboratory Analysis logoLink to Journal of Clinical Laboratory Analysis
. 1998 Dec 7;11(3):154–157. doi: 10.1002/(SICI)1098-2825(1997)11:3<154::AID-JCLA6>3.0.CO;2-A

Specific recognition of hydatid cyst antigens by serum IgG, IgA using Western blot

Younes Sbihi 1, Dirk Janssen 1, Antonio Osuna 1,✉
PMCID: PMC6760729  PMID: 9138104

Abstract

Diagnosis of hydatid disease in humans relies on the detection of specific antibodies against antigens of the matacestode from Echinococcus granulosus. The specificity and sensitivity of current immunological techniques based on specific serum IgG rely on the way antigens are purified. We used Western immunoblotting to detect specific IgG, IgE, and IgA antibodies in serum from patients with hydatid disease using either crude antigen preparations (total hydatid fluid), purified fractions enriched in Antigens 5 and B, and glycoproteins from hydatid fluid. Depending on whether crude HF or purified antigen fractions were used, IgG and IgE recognized specifically low‐to‐medium MW bands between 12 and 42 kDa. IgA recognized specifically 110 kDa band in crude hydatid fluid and in the glycoprotein fraction of hydatid fluid, and a 42 kDa band in all antigen samples used. Besides the advantage of detecting specific IgA in crude hydatid fluid, these results offer the possibility of simplifying future immunological tests if specific secretory IgA can be similarly detected. J. Clin. Lab. Anal. 11:154–157, 1997. © 1997 Wiley‐Liss, Inc.

Keywords: Echinococcus granulosus, hydatid disease, Western blotting, IgE, IgG, IgA, antigen

References

  • 1. Hira PR, Shweiki HM, Francis I: Cystic hydatid disease—Pitfalls in diagnosis in the Middle East endemic area. J Trop Med Hyg 96: 363–369, 1993. [PubMed] [Google Scholar]
  • 2. Babba H, Messedi A, Masmoudi S, Zribi M, Grillot R, Ambriose‐Thomas P, Beyrouti I, Sahnoun Y: Diagnosis of human hydatidosis—Comparison between imagery and 6 serologic techniques. Am J Trop Med Hyg 50: 64–68, 1994. [DOI] [PubMed] [Google Scholar]
  • 3. Lightowlers MW, Gottstein B: Echinococcosis/hydatidosis: Antigens, immunological and molecular diagnosis In Echinococcus and Hydatid Disease, Thompson RCA, Lymbery AJ, eds. Cab International, Oxon, 1995, p 376–380. [Google Scholar]
  • 4. Shepherd JC, McManus DP: Specific and cross‐reactive antigens of Echinococcus granulosus cyst fluid. Mol Biochem Parasitol 25: 143–154, 1987. [DOI] [PubMed] [Google Scholar]
  • 5. Sbihi Y, Janssen D, Osuna A: Serologic recognition of hydatid cyst antigens using different purification methods. Diagn Microbiol Infect Dis, 1996 24: 205–211, 1996. [DOI] [PubMed] [Google Scholar]
  • 6. Matossian RM, Añami SY, Salti I, Araj GF: Serum immunoglobulin levels in human hydatidosis. Int J Parasitol 6: 367–371, 1976. [DOI] [PubMed] [Google Scholar]
  • 7. Richard‐Lenoble D, Smith MD, Loiuy M, Verdoust PJ: Human hydatidosis: Evaluation of three serodiagnostic methods, the principal subclass of specific immunoglobulin and detection of circulating immune complexes. Am Trop Med Parasitol 72: 553–560, 1978. [DOI] [PubMed] [Google Scholar]
  • 8. Rogan MT, Craig PS, Zeyhle E, Romig T, Lubano GM, Deshan L: Evaluation of a rapid dot‐ELISA as a field test for the diagnosis of cystic hydatid disease. Trans Roy Soc Trop Med Hyg 85: 773–777, 1991. [DOI] [PubMed] [Google Scholar]
  • 9. Oriol R, Williams JF, Pérez Esandi MV, Oriol C: Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. Am J Trop Med Hyg 20: 569–574, 1971. [DOI] [PubMed] [Google Scholar]
  • 10. Kennedy JF, Rosevear A: An assessment of the fractionation of carbohydrate on Concanavalin A Sepharose 4B by affinity chromatography. J Chem Soc Perkin Trans 19: 2041–2046, 1973. [DOI] [PubMed] [Google Scholar]
  • 11. Findlay JBC: The isolation and labelling of membrane proteins and peptides In Biological Membranes: A Pratical Approach, Findlay JBC, Houck JC, eds. IRL Press, Oxford, 1986, p 179–213. [Google Scholar]
  • 12. Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354, 1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13. Voller A, Bidwell D: Enzyme‐linked Immunosorbent Assay In Manual of Clinical Laboratory Immunology, Rose NR, Feriedman H, Fahey JL, eds. American Society for Microbiology, Washington, DC, 1986, p 107. [Google Scholar]
  • 14. Schantz PM, Shanks D, Wilson M: Nonspecific reactions with the intradermal test for hydatidosis in persons with other helminth infections. Am J Trop Med Hyg 24: 849–852, 1980. [DOI] [PubMed] [Google Scholar]
  • 15. Kagan IG, Agosin M: Echinococcus antigens. Bull WHO 39: 13–24, 1968. [PMC free article] [PubMed] [Google Scholar]
  • 16. Pozzuoli RM, Piantelli M, Perucci C, Arru E, Musiaji P: Isolation of the most immunoreactive antigens of Echinococcus granulosus from sheep hydatid fluid. J Immunol 115: 1459–1463, 1975. [PubMed] [Google Scholar]
  • 17. Williams JF: Hydatidosis/cysticercosis: Immune mechanisms and immunization against infection. Adv Parasitol 21: 229–295, 1982. [DOI] [PubMed] [Google Scholar]
  • 18. Bekhti A, Schaap JP, Capton M, Dessaint JP, Santoro F, Capron A: Treatment of hepatic hydatid disease with mebendazole: preliminary results in four cases. Br Med J 2: 1047–1051, 1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19. Dessaint JP, Bout D, Wattre P, Capron A: Quantitative determination of specific IgE antibodies to Echinococcus granulosus and IgE levels in sera from patients with hydatid disease. Immunology 29: 813–823, 1975. [PMC free article] [PubMed] [Google Scholar]
  • 20. Di Candilo F, Papili R, Francisi D, Preziosi R, Marroni M, Menichetti F, Baldelli F: IgE amplified ELISA in the diagnosis of human hydatidosis In XV Extraordinary Congress for the Celebration of the 50 years of A.I.H., De Rosa F, ed. John Libbey CIC s.r.l., Rome: 1991, p 971–975. [Google Scholar]
  • 21. Schantz PM, Shanks D, Wilson M: Serologic cross‐reactions with sera from patients with echinococcosis and cysticerosis. Am J Trop Med Hyg 29: 609–612, 1980. [DOI] [PubMed] [Google Scholar]
  • 22. Shweiki HM, Bahr GM, Salama MS, Behbehani K, Hira PR: Analysis of the in vitro lymphoproliferative responses and antibody levels of the arc‐5 antigen in patients with cystic hydatic disease. Ann Trop Med Parasitol 86: 621–629, 1992. [DOI] [PubMed] [Google Scholar]
  • 23. Tomasi TB, Bienenstock J: Secretory immunoglobulins. Adv Immunol 9: 1, 1968. [DOI] [PubMed] [Google Scholar]
  • 24. Lloyd S, Soulsby EJL: Immunological responses of the host In Parasitology in Focus, Melhorn H, ed. Springer‐Verlag, Berlin, 1988, p 619–650. [Google Scholar]
  • 25. Lightowlers MW, Liu D, Haralambous A, Rickard MD: Subunit composition and specificity of the major cyst fluid antigens of Echinococcus granulosus. Mol Biochem Parasitol 37: 171–182, 1989. [DOI] [PubMed] [Google Scholar]
  • 26. Siracusano A, Ioppolo S, Notargiacomo S, Ortona E, Rigano R, Teggi A, De Rosa F, Vicari G: Detection of antibodies against Echinococcus granulosus major antigens and their subunits by immunoblotting. Trans Roy Soc Trop Med Hyg 85: 239–243, 1991. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Laboratory Analysis are provided here courtesy of Wiley

RESOURCES