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. 1998 Dec 7;11(2):110–116. doi: 10.1002/(SICI)1098-2825(1997)11:2<110::AID-JCLA7>3.0.CO;2-F

Significance of urinary type IV collagen in patients with diabetic nephropathy using a highly sensitive one‐step sandwich enzyme immunoassay

Mitsunori Yagame 1,✉, Daisuke Suzuki 1, Kiichiro Jinde 1, Noboru Saotome 1, Haruhiro Sato 1, Masako Noguchi 1, Hideto Sakai 1, Takao Kuramoto 2, Keiko Sekizuka 2, Toshihiko Iijima 3, Shigenobu Suzuki 3, Yasuhiko Tomino 1
PMCID: PMC6760738  PMID: 9058245

Abstract

Urinary concentrations of type IV collagen in patients with diabetic nephropathy were measured by a highly sensitive, one‐step sandwich enzyme immunoassay. Samples from 298 patients with non‐insulin‐dependent diabetes mellitus (NIDDM) and 80 healthy controls were examined. In diabetic patients with macroalbuminuria or renal insufficiency, the concentrations of urinary type IV collagen were significantly higher than those of diabetic patients with normoalbuminuria or healthy controls (P < 0.001). Urinary type IV collagen concentration in diabetic patients with microalbuminuria was significantly higher than that in diabetic patients with normoalbuminuria or that in healthy controls (P < 0.001). In contrast, there were no significant changes in the concentration of serum type IV collagen between microalbuminuric patients and normoalbuminuric patients. The area under the receiver operating characteristic (ROD) curve for the urinary type IV collagen concentration was equivalent to that of urinary albumin. It was concluded that urinary type IV collagen concentration determined using this method might be a useful marker for the early detection of diabetic nephropathy. J. Clin. Lab. Anal. 11:110–116. © 1997 Wiley‐Liss, Inc.

Keywords: diabetic nephropathy, non insulin‐dependent diabetes mellitus (NIDDM), type IV collagen, receiver operating characteristic analysis, microalbuminuria

References

  • 1. Hayashi H, Karasawa R, Inn H, Saitou T, Ueno M, Nishi S, Suzuki Y, Ogino S, Maruyama Y, Kouda Y, Arakawa M: An electron microscopic study of glomeruli in Japanese patients with non‐insulin dependent diabetes mellitus. Kidney Int 41: 749–757, 1992. [DOI] [PubMed] [Google Scholar]
  • 2. Mauer SM, Steffes MW, Ellis EN, Sutherland DER, Brown DM, Goetz FC: Structural‐functional relationships in diabetic nephropathy. J Clin Invest 74: 1143–1155, 1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3. Steffes MW, Østerby R, Chavers B, Mauer SM: Mesangial expansion as a central mechanism for loss of kidney function in diabetic patients. Diabetes 38: 1077–1081, 1989. [DOI] [PubMed] [Google Scholar]
  • 4. Steffes MW, Bilous RW, Sutherland DER, Mauer SM: Cell and matrix components of the glomerular mesangium in type I diabetes mellitus. Diabetes 41: 679–684, 1992. [DOI] [PubMed] [Google Scholar]
  • 5. Högemann B, Balleisen L, Rauterberg J, Voss B, Gerlach U: Basement membrane components (7S collagen, laminine P1) are increased in sera of diabetic and activate platelets in vitro. Haemostasis 16: 428–432, 1986. [DOI] [PubMed] [Google Scholar]
  • 6. Hayashi Y, Makino H, Ota Z: Serum and urinary concentrations of type IV collagen and laminin as a marker of microangiopathy in diabetes. Diabetic Med 9: 366–370, 1992. [DOI] [PubMed] [Google Scholar]
  • 7. Matsumoto E, Matsumoto G, Ooshima A, Kikuoka H, Bessho H, Miyamura K, Nanjo K: Serum type IV collagen concentrations in diabetic patients with microangiopathy as determined by enzyme immunoassay with monoclonal antibodies. Diabetes 39: 885–890, 1990. [DOI] [PubMed] [Google Scholar]
  • 8. Kuzuya N: Report of the committee of diagnostic criteria of diabetes mellitus by the glucose tolerance test. J Jpn Diab Soc 13: 17–20, 1970. [Google Scholar]
  • 9. Mayne R, Zettergren JG, Mayne PM, Bedwell NW: Isolation and partial characterization of basement membrane‐like collagens from bovine thoracic aorta. Artery 7: 262–280, 1980. [PubMed] [Google Scholar]
  • 10. Matsumoto E: Foreknowledge of liver fibrosis: Development of immunoassay system for serum collagen peptides monoclonal antibody. J Wakayama Med Soc 39: 87–106, 1988. [Google Scholar]
  • 11. Baum SG, Horwitz MS, Maizel JV Jr: Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40. J Virol 10: 211–219, 1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12. Hashida S, Imagawa M, Inoue S, Ruan K‐H, Ishikawa E: More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge. J Appl Biochem 6: 56–63, 1984. [PubMed] [Google Scholar]
  • 13. Ishikawa E, Imagawa M, Mashida S, Hamaguchi Y, Ueno T: Enzymelabeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining. J Immunoassay 4: 209–327, 1983. [DOI] [PubMed] [Google Scholar]
  • 14. Bos ES, Van der Doelen AA, Rooy NV, Schuurs AHWM: 3,3′5,5′‐Tetramethylbenzidine as an Ames test negative chromogen for horseradish peroxidase in enzyme‐immunoassay. J Immunoassay 2: 187–204, 1981. [DOI] [PubMed] [Google Scholar]
  • 15. Hanley JA, McNeil BJ: The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology 143: 29–36, 1982. [DOI] [PubMed] [Google Scholar]
  • 16. Hanley JA, McNeil BJ: A method of comparing the areas under receiver operating characteristic curves derived from same cases. Radiology 148: 839–843, 1983. [DOI] [PubMed] [Google Scholar]
  • 17. Shigeta Y, Kikkawa R: Diabetic nephropathy in Japan. Diabetes Res and Clin Practice 24(Suppl): S191–S197, 1994. [DOI] [PubMed] [Google Scholar]
  • 18. Yagame M, Kim Y, Zhu D, Suzuki D, Eguchi K, Nomoto Y, Sakai H, Groppoli T, Steffes MW, Mauer SM: Differential distribution of type IV collagen chains in patients with diabetic nephropathy in non‐insulindependent diabetes mellitus. Nephron 70: 42–48, 1995. [DOI] [PubMed] [Google Scholar]
  • 19. Suzuki D: Measurement of the extracellular matrix in glomeruli from patients with diabetic nephropathy using an antomatic image analyzer. Jpn J Nephrol 36: 1209–1215, 1994. [PubMed] [Google Scholar]
  • 20. Suzuki Y: Constituents of the extracellular matrices in diabetic glomerulosclerosis. Jpn J Nephrol 31: 1047–1054, 1989. [PubMed] [Google Scholar]
  • 21. Nerlich A, Schleicher E: Immunohistochemical localization of extracellular matrix components in human diabetic glomerular lesions. Am J Pathol 139: 889–899, 1991. [PMC free article] [PubMed] [Google Scholar]
  • 22. Mohan PR, Carter WG, Spiro RG: Occurrence of type IV collagen in extracellular matrix of renal glomeruli and its increase in diabetes. Diabetes 39: 31–37, 1990. [DOI] [PubMed] [Google Scholar]
  • 23. Falk RJ, Scheinman JI, Mauer SM, Michael AF: Polyantigenic expansion of basement membrane constituents in diabetic nephropathy. Diabetes 32(Suppl 2): 34–39, 1983. [DOI] [PubMed] [Google Scholar]
  • 24. Haneda M, Kikkawa R, Horide N, Togawa M, Koya D, Kajiwara N, Ooshima A, Shigeta Y: Glucose enhances type IV collagen production in cultured rat glomerular mesangial cells. Diabetologia 34: 198–200, 1991. [DOI] [PubMed] [Google Scholar]
  • 25. Ayo SH, Radnik RA, Glass WF II, Garoni JA, Rampt ER, Appling DR, Kreisberg JI: Increased extracellular matrix synthesis and mRNA in mesangial cells growth in high‐glucose medium. Am J Physiol 260: F185–F191, 1991. [DOI] [PubMed] [Google Scholar]
  • 26. Kitamura M, Kitamura A, Mitarai T, Maruyama N, Nagasawa R, Kawamura T, Yoshida H, Takahashi T, Sakai O: Gene expression of metalloproteinase and its inhibitor in mesangial cells exposed to high glucose. Biochem Biophys Res Commun 185: 1048–1054, 1992. [DOI] [PubMed] [Google Scholar]
  • 27. Doi T, Vlassara H, Kirstein M, Yamada Y, Striker GE, Striker LJ: Receptor‐specific increase in extramatrix production in mouse mesangial cells by advanced glycosylation end products is mediated via platelet‐derived growth factor. Proc Natl Acad Sci USA 89: 2873–2877, 1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28. Harries RC, Haralson MA, Badr KF: Continuous stretch‐relaxation in culture alters rat mesangial cell morphology, growth characteristics and metabolic activity. Lab Invest 66: 548–554, 1992. [PubMed] [Google Scholar]
  • 29. Wakisaka M, Spiro MJ, Spiro RG: Synthesis of type IV collagen by cultured glomerular cells and comparison of its regulation by glucose and other factors with that of type IV collagen. Diabetes 43: 95–103, 1994. [DOI] [PubMed] [Google Scholar]
  • 30. Ziyadeh FN, Cohen MP: Effects of glycated albumin on mesangial cells: Evidence for a role in diabetic nephropathy. Mol Cell Biochem 125: 19–25, 1993. [DOI] [PubMed] [Google Scholar]
  • 31. Ayo SH, Radnik RA, Garoni JA, Glass F II, Kreisberg JI: High glucose causes an increase in extracellular matrix proteins in cultured mesangial cells. Am J Pathol 136: 1339–1348, 1990. [PMC free article] [PubMed] [Google Scholar]
  • 32. Danne T, Spiro MJ, Spiro RG: Effect of high glucose on type IV collagen production by cultured glomerular epithelial, endothelial, and mesangial cells. Diabetes 42: 170–177, 1993. [DOI] [PubMed] [Google Scholar]
  • 33. Ziyadeh FN, Simmons DA, Snipes ER, Goldfarb S: Effect of myo‐inositol on cell proliferation and collagen transcription and secretion in proximal tubule cells cultured in elevated glucose. J Am Soc Nephrol 1: 1220–1229, 1991. [DOI] [PubMed] [Google Scholar]
  • 34. Rocco MV, Chen Y, Goldfarb S, Ziyadeh FN: Elevated glucose stimulate TGF‐β gene expression and bioactivity in proximal tubule. Kidney Int 41: 107–114, 1992. [DOI] [PubMed] [Google Scholar]
  • 35. Ikeda S, Haramoto T, Shikata K, Kumagai I, Makino H, Ota Z: Changes in glomerular basement membrane components in diabetic nephropathy. Connective Tissue 21: 103–104, 1990. [Google Scholar]
  • 36. Kim Y, Kleppel MM, Butkowski R, Mauer SM, Wieslander J, Michael AF: Differential expression of basement membrane collagen chains in diabetic nephropathy. Am J Pathol 138: 413–420, 1991. [PMC free article] [PubMed] [Google Scholar]
  • 37. Schleicher ED, Olgemöller B: Glomerular change in diabetes mellitus. Eur J Clin Chem Clin Biochem 30: 635–640, 1992. [PubMed] [Google Scholar]
  • 38. Jinde K, Yagame M, Suzuki D, Naka R, Sakai H, Miyazaki M: In situ hybridization of MMP‐3 and TIMP‐1 mRNA in glomeruli from noninsulin‐dependent diabetes mellitus patients with diabetic nephropathy. Abstract of the XIIIth International Congress of Nephrology, 195, 1995.

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