Fig. 3.

A: SDS-PAGE of purified, concentrated TerS-eGFP and TerS-mCherry with Western blotting using gp16 antiserum for TerS identification and showing fluorescence of the purified concentrated fusion proteins. The sizes and purities of the boiled SDS buffer loaded protein are compared to rainbow marker size protein standards. B: Superose 6 size exclusion column chromatography profile of 50 μl of purified concentrated T4 TerS-eGFP (see Fig. 3A) plus 50 μl Biorad Gel filtration protein size standards with Typhoon assessed fluorescence (upper left inset). The identified four protein chromatography traces show 260nm, purple, 280nm green, 405nm orange, and 214nm gray wavelength absorptions of the size standards labeled in the figure. Size standards alone do not show the identified TerS-eGFP peak fluorescence or imidazole peak; fractions 3–31 are equal 0.2 ml volume column fractions; TerS-eGFP is found in fractions 3–13, ≥ 670 kDa. Left Y-axis provides the optical densities at the four wavelengths listed, top X-axis the fraction collector tube number.