Skip to main content
. 2019 Sep 16;8:e46773. doi: 10.7554/eLife.46773

Figure 2. Nxph4 KO mice displayed multiple neurological deficits.

(A) Plots of weight as a function of age (male n = 13, female n = 16–18; #, difference between HET and KO; * difference between WT and KO). (B) Latency to fall from the accelerating rotarod plotted as a function of training days (male n = 10–12, female n = 12). (C) Average time spent in the open arms of the elevated plus maze (male n = 13–17, female n = 12–14). (D) Mean of response to the 120 dB acoustic stimulus (male n = 13–15, female n = 12–14). (E) Pre-pulse inhibition at 74 dB, 78 dB and 82 dB pre-pulses (male n = 13–15, female n = 12–14). Data are presented as mean ± SEM. *, # p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; by one-way or two-way ANOVA.

Figure 2.

Figure 2—figure supplement 1. Nxph4 KO mice gained less weight but have normal locomotor and righting reflex functions.

Figure 2—figure supplement 1.

(A) RNA in situ hybridization with a probe against Nxph4 only detected Nxph4 signal in WT but not KO mice. Scale bars: 2 mm. (B) RT-qPCR from cerebellar samples with specific primers for Nxph4 (n = 3). (C, D) Weight of male mice at 9 months of age and female mice at 13 months of age (male n = 14–18, female n = 10). (E, F) Total distance traveled in the open field assay (male n = 12–14, female n = 16–17). (G, H) Righting reflex assay was performed on postnatal day 10 mice. Y-axis shows the time that mice spent to reflex themselves after being placed on their back (male n = 11–28, female n = 11–29). Data are presented as mean ± SEM. *, p<0.05; **, p<0.01; n.s., not significant; by one-way ANOVA.