Molecular Heterogeneity of the Voltage-Gated Fast Transient Outward K⁺ Current, I_Af, in Mammalian Neurons

Abstract

Recently, we identified four kinetically distinct voltage-gated K⁺ currents, I_Af,I_As,I_K, andI_SS, in rat superior cervical ganglion (SCG) neurons and demonstrated thatI_Af and I_As are differentially expressed in type I (I_Af,I_K,I_SS), type II (I_Af,I_As,I_K,I_SS), and type III (I_K,I_SS) SCG cells. In addition, we reported that I_Af is eliminated in most (∼70%) SCG cells expressing Kv4.2W362F, a Kv4 subfamily-specific dominant negative. The molecular correlate(s) of the residualI_Af, as well as that ofI_As,I_K, andI_SS, however, are unknown. The experiments here were undertaken to explore the role of Kv1 α-subunits in the generation of voltage-gated K⁺currents in SCG neurons. Using the Biolistics Gene Gun, cDNA constructs encoding a Kv1 subfamily-specific dominant negative, Kv1.5W461F, and enhanced green fluorescent protein (EGFP) were introduced into SCG neurons. Whole-cell recordings from EGFP-positive Kv1.5W461F-expressing cells revealed a selective decrease in the percentage of type I cells and an increase in type III cells, indicating that I_Af is gated by Kv1 α-subunits in a subset of type I SCG neurons.I_Af is eliminated in all SCG cells expressing both Kv1.5W461F and Kv4.2W362F.I_Af τ_decay values in Kv1.5W461F-expressing and Kv4.2W362F-expressing type I cells are significantly different, revealing that Kv1 and Kv4 α-subunits encode kinetically distinct I_Af channels. Expression of Kv1.5W461F increases excitability by decreasing action potential current thresholds and converts phasic cells to adapting or tonic firing. Interestingly, the molecular heterogeneity ofI_Af channels has functional significance because Kv1- and Kv4-encoded I_Af play distinct roles in the regulation of neuronal excitability.

K⁺channels are important determinants of membrane excitability, and differences in K⁺ channel expression influence action potential waveforms, repetitive firing patterns, and synaptic integration (Pongs, 1999; Hausser et al., 2000). Electrophysiological studies have revealed that most mammalian neurons express multiple types of voltage-gated K⁺current components with distinct time- and voltage-dependent properties (Rudy, 1988; Storm, 1990). In addition, a number of voltage-gated K⁺ (Kv) channel pore-forming (α) and accessory (Kvβ and KChIP) subunits that underlie these channels have been identified (Coetzee et al., 1999; Pongs, 1999; An et al., 2000), and there is presently considerable interest in identifying the molecular correlates and defining the functional roles of the various voltage-gated K⁺ currents in mammalian neurons.

Recently, we reported that sympathetic neurons isolated from the rat superior cervical ganglion (SCG) express multiple, kinetically distinct, voltage-gated K⁺ current components: two transient A-type currents,I_Af andI_As; a delayed rectifier current,I_K; and a steady-state non-inactivating current, I_SS (Malin and Nerbonne, 2000). Although I_K andI_SS are present in all rat SCG neurons, I_Af andI_As are differentially distributed;I_Af is detected in ∼90% of cells and, in a subset of these cells (∼20%),I_As is also expressed (Malin and Nerbonne, 2000). Based on these expression patterns, three cell types were distinguished: type I cells (I_Af,I_K,I_SS), type II cells (I_Af,I_As,I_K,I_SS), and type III cells (I_K,I_SS) (Malin and Nerbonne, 2000). In addition, we exploited a molecular genetic strategy to assess directly the role of Kv4 α-subunits in the generation of voltage-gated K⁺ currents in these cells. These experiments revealed that expression of Kv4.2W362F, a Kv4 subfamily-specific dominant negative (Barry et al., 1998), results in the elimination of I_Af in most (∼70%) SCG cells (Malin and Nerbonne, 2000). The molecular correlate(s) of the residual I_Af, as well as of I_As,I_K, andI_SS, have not been defined.

Previous studies have documented the expression of mRNAs encoding several Kv α- and β-subunits in SCG neurons, including several members of the Kv1 subfamily, Kv1.1, Kv1.2, Kv1.4, and Kv1.5 (Dixon and McKinnon, 1996; Pankevych et al., 1999). Interestingly, previous studies have shown that heterologous expression of Kv1 α-subunits alone or in combination with Kvβ subunits gives rise to voltage-gated K⁺ currents with a wide range of time- and voltage-dependent properties (Coetzee et al., 1999). In addition, it has recently been demonstrated that Kv1 α-subunits encode distinct types of voltage-gated K⁺ currents in myocardial cells (Feng et al., 1997; Bou-Abboud and Nerbonne, 1999; Guo et al., 1999, 2000; London et al., 2001). In mouse ventricular myocytes, for example, Kv1.4 encodes the slow transient K⁺ current,I_to,s (Guo et al., 1999, 2000), and Kv1.5 underlies one component of the slow delayed rectifier currentI_K,slow (London et al., 2001). These observations led us to hypothesize that Kv1 α-subunits might contribute to the formation of one or more voltage-gated K⁺ currents in rat SCG neurons, and the experiments here were undertaken to test this hypothesis directly.

MATERIALS AND METHODS

SCG neurons in vitro. Sympathetic neurons were isolated from the SCGs of embryonic day 21 to postnatal day 1 Long–Evans rat pups using a procedure similar to that described by Chang et al. (1990). Briefly, after anesthesia with 5% halothane, animals were decapitated, and the SCGs were removed. Ganglia were successively incubated for 30 min periods in collagenase and trypsin at room temperature; isolated SCG neurons were obtained by trituration and subsequent centrifugation. Dissociated SCG cells were resuspended in growth medium (Earle's Minimum Essential Medium with 10% fetal calf serum, 0.14 mml-glutamine, 100 U/ml penicillin–streptomycin, and 0.05 mm NGF) and plated at a density of 2.5 × 10⁴ cells per square centimeter on glial monolayers (prepared as in Raff et al., 1979). Cells were maintained at 37°C in a 95% O₂/5% CO₂ incubator, and the medium was exchanged with fresh growth medium approximately every 48 hr.

Immunohistochemistry. The affinity-purified rabbit polyclonal anti-Kv1.2 antibody used here was raised against residues 468–486 in the C terminus of Kv1.2 and has been shown to reliably and specifically detect Kv1.2 (Barry et al., 1995). The Kv1.4-specific antibody was targeted against residues 13–37 in the N-terminal region of Kv1.4 and has been shown previously to detect Kv1.4 with no detectable cross-reactivity to other Kv1 subfamily members (Barry et al., 1995). The anti-Kv1.5 antibody, purchased from UBI (Lake Placid, NY; catalog #06-463), was generated against amino acid residues 542–602 in the C terminus of Kv1.5 and has been demonstrated to be specific for Kv1.5 (Barry et al., 1995). For immunohistochemistry, cells were fixed in 4% paraformaldehyde for 30 min, incubated in blocking buffer (PBS containing 5% normal goat serum, 0.02% Triton X-100, and 0.1% NaNH₃) for 1 hr, and exposed to one of the Kv1 α-subunit-specific primary antibodies (1:100 dilution) at 4°C overnight. After washing with PBS, cultures were incubated first with a biotinylated goat anti-rabbit antibody (1:200 dilution; Chemicon, Temecula, CA) for 1 hr at room temperature, washed again, and then exposed to an avidin-conjugated horseradish peroxidase for 1 hr at room temperature (ABC kit; Vector Laboratories, Burlingame, CA). The cultures were washed again before incubation with 0.05% diaminobenzidine in PBS. The progress of the reaction was monitored under the microscope, and the reactions were quenched after ∼5 min by washing with PBS. A mouse monoclonal anti-myc antibody (Calbiochem, La Jolla, CA) was used at a 1:100 dilution to detect Kv1.5W461F-myc protein expression in transfected SCG neurons. Cultures were fixed, blocked, and incubated in primary antibody as described above. After washing, cultures were incubated with a Cy3-conjugated rabbit anti-mouse IgG secondary antibody (Chemicon), and labeled cells were visualized under epifluorescence illumination.

Transfection of isolated SCG neurons. In control experiments, 1.6 μm gold beads were coated with pCMV-EGFP (Clontech, Cambridge, UK) and propelled (450 psi; 2 mm carrier distance) into SCG neurons at 4 d in vitro using the Gene Gun (Bio-Rad, Hercules, CA), a biolistic projectile system. After transfections, the cultures appeared healthy, and expression of EGFP was readily detected 24 hr later (see Results). In experiments aimed at examining the effects of Kv1.4, Kv1.5W461F or Kv4.2W362F + Kv1.5W461F expression in SCG neurons, the gold particles were coated with pBK-CMV-Kv1.4 and pCMV-EGFP (4:1 ratio); pBK-CMV-Kv1.5W461F-myc and pCMV-EGFP (4:1 ratio); or pBK-CMV-Kv4.2W362F-FLAG, pBK-CMV-Kv1.5W461F-myc and pCMV-EGFP (4:4:1 ratio). In all cases, a total of 10 μg of DNA was used in coating the beads. Because EGFP expression was used to identify transfected neurons for subsequent electrophysiological characterization, immunohistochemical experiments were performed to determine whether EGFP-expressing cells also expressed the Kv1.5W461F-myc construct (see Results).

Electrophysiological recording. Whole-cell recordings were obtained from isolated SCG neurons at room temperature (22–25°C). Data were collected using an Axopatch-1B patch-clamp amplifier, and experiments were controlled with a P5–120 Gateway 2000 computer through a TL-1 DMA interface using the pClamp7 software package (Axon Instruments, Foster City, CA). Electrodes were fabricated from soda-lime glass (Chase 2502) with a two-stage puller, and the shanks were coated with a silicone elastomer (Sylgard; Dow Corning, Midland, MI). Pipette resistances were 1.5–3 MΩ after fire-polishing. For voltage-clamp recordings, the bath solution routinely contained (in mm): 140 NaCl, 4 KCl, 2 CaCl₂, 2 MgCl₂, 10 HEPES, 5 glucose, 0.001 TTX, and 0.1 CdCl₂, pH 7.5, 300 mOsm. Recordings were also obtained from SCG neurons in the current-clamp mode, and the TTX and CdCl₂ were omitted from the bath for these studies. The pipette solution for both current- and voltage-clamp recordings contained (in mm): 135 KCl, 10 HEPES, 5 glucose, 3 MgATP, 0.5 NaATP, 2 EGTA, 1.1 CaCl₂, pH 7.5, 300 mOsm. Series resistances, estimated from the decays of the uncompensated capacitative transients, were 2–5 MΩ and were compensated electronically by ∼80–90%. Because current amplitudes were <10 nA, the voltage errors resulting from the uncompensated series resistance were always <10 mV and were not corrected. Voltage-gated K⁺ currents were evoked during 125 msec or 6 sec depolarizing voltage steps to test potentials between −40 mV and +50 mV from a holding potential of −90 mV. Single action potentials and action potential trains were recorded in response to brief (1.5 msec) 200–400 pA and prolonged (500 msec) 20–200 pA depolarizing current injections.

Data analysis. Data were compiled and analyzed using pClamp7 and Excel (Microsoft, Seattle, WA) software and are presented as means ± SEM. The decay phases of the capacitative transients were analyzed, and only cells in which >90% of the amplitude of the capacitative transient decayed with a single exponential time course were included in this study. Only data obtained from cells with input resistances >100 MΩ were analyzed and included here. The mean ± SEM input resistance and capacitance of EGFP-expressing SCG neurons were 0.34 ± 0.03 GΩ and 32 ± 2 pF (n = 43), respectively. Leak currents were <100 pA (at −70 mV) and were not subtracted. To determine the amplitudes and the decay time constants of the individual components of the total depolarization-activated outward K⁺currents in SCG neurons, the inactivation phases of the currents recorded during prolonged (6 sec) depolarizations were analyzed using the equation: y =A₁e^−t/τ1+A₂e^−t/τ2+A₃e^−t/τ3+ C, where A₁,A₂, andA₃ (measured in picoamperes per picoFarad) are the amplitudes of the inactivating current components (I_Af,I_As, andI_K) that decay with time constants τ₁, τ₂, and τ₃ (measured in milliseconds), respectively, and C is the steady-state current (measured in picoamperes per picoFarad) remaining at the end of the 6 sec depolarizations (see Results). Fits were obtained using CLAMPFIT6, and best fits were determined by eye (in all cases, ς < 30 pA). All current-clamp recordings were obtained from cells with overshooting action potentials and stable resting membrane potentials negative to −40 mV. Action potential durations were measured at 50% (APD₅₀) and 90% (APD₉₀) repolarization. Statistical significance was examined with the Student's t test; where appropriate, p values are presented in the text.

RESULTS

Heterogeneity of outward K⁺ current waveforms in SCG neurons

Previously, we have shown that the waveforms of the Ca²⁺-independent, depolarization-activated K⁺ currents in isolated rat SCG neurons are highly stereotyped (Malin and Nerbonne, 2000). The records presented in Figure 1, left panel, are representative of the three distinct phenotypes observed; they are referred to as types I, II, and III. Most (>90%) wild-type SCG cells express a prominent rapid component of current decay, consistent with the presence of the fast transient “A-type” current, referred to asI_Af (Malin and Nerbonne, 2000). The exception is type III cells, which express only a slowly decaying outward K⁺ current,I_K, and a non-inactivating current,I_SS (Fig. 1, left panel; Table 1). In type I SCG cells (Fig. 1,left panel), the decay phases of the currents are well fitted by the sum of two exponentials, reflecting the presence of I_Af (mean ± SEM τ_decay = 121 ± 14 msec),I_K (mean ± SEM τ_decay = 2560 ± 187 msec), and the non-inactivating current I_SS (Table1). In addition to I_Af,I_K, andI_SS, a more slowly inactivating, transient outward K⁺ current, referred to as I_As, (mean ± SEM τ_decay 480 ± 21 msec) is present in type II SCG cells (Fig. 1, left panel; Table 1). The decay properties of the individual current components are similar in types I, II, and III SCG cells, although there are marked differences in current densities (Table 1).

Fig. 1.

Distinct effects of expression of Kv4.2W362F or Kv1.5W461F on voltage-gated outward K⁺currents in SCG neurons. Whole-cell voltage-gated outward K⁺ currents were recorded from isolated SCG neurons in response to 6 sec depolarizing voltage steps to test potentials between −10 and +50 mV from a holding potential of −90 mV. Experiments were conducted as described in Materials and Methods with 1 μm TTX and 100 μm CdCl₂ in the bath solution to block voltage-gated inward Na⁺ and Ca²⁺ currents, respectively. The records in theleft, middle, and right panels were recorded from wild-type, Kv4.2W362F-expressing, and Kv1.5W461F-expressing cells, respectively. There are distinct and stereotyped differences in the waveforms of the currents in wild-type I, II, and III SCG cells (see Results). There is, for example, a prominent rapid component of current decay,I_Af, in type I and II cells that is not evident in type III cells. The numbers given above the records in each column reflect the percentages of cells studied under each experimental condition that display the type I, II, or III phenotype. Expression of either Kv4.2W362F (middle) or Kv1.5W461F (right) decreases the percentage of type I cells and increases the percentage of type III cells. Kv4.2W362F expression also eliminatesI_Af in type II cells (see Results) (Malin and Nerbonne, 2000).

Table 1.

Outward K⁺ currents in wild-type SCG neurons expressing Kv4.2W362F and/or Kv1.5W461F ^1-a

	Wild type			Kv4.2W362F1-b			Kv1.5W461F			Kv1.5W461F + Kv4.2W362F
	Type I	Type II	Type III	Type I	Type II	Type III	Type I	Type II	Type III	Type II	Type III
Peak current density (pA/pF)	252  ± 20	259  ± 22	211  ± 18	244  ± 35	289  ± 31	162  ± 21	241  ± 22	260  ± 33	239  ± 28	219  ± 42	218  ± 35
IAf
Density (pA/pF)	81  ± 11	111  ± 21		64  ± 20			67  ± 6	83  ± 9
τ (msec)	121  ± 14	95  ± 8		190  ± 18*			97  ± 101-160	72  ± 8
IAs
Density (pA/pF)		45  ± 3			67  ± 15			40  ± 8		56  ± 14
τ (msec)		480  ± 21			490  ± 31			585  ± 47		482  ± 61
IK
Density (pA/pF)	108  ± 12	64  ± 9	136  ± 11	115  ± 7	157  ± 25	111  ± 22	90  ± 13	74  ± 10	171  ± 36	102  ± 44	135  ± 60
τ (msec)	2560  ± 187	2800  ± 193	2200  ± 176	2368  ± 197	2473  ± 254	2050  ± 235	3127  ± 197	3125  ± 438	3089  ± 234	2789  ± 363	2473  ± 254
ISS
Density (pA/pF)	74  ± 5	45  ± 5	79  ± 6	68  ± 15	72  ± 15	65  ± 9	52  ± 8	48  ± 6	84  ± 7	65  ± 9	86  ± 12
n	30	9	4	6	7	18	15	8	11	3	10

^F1-aAll values are mean ± SEM; current densities and τ_decay values were determined from currents recorded on depolarizations to +50 mV; n= number of cells.

^F1-bData from Malin and Nerbonne (2000).

^*Values in Kv4.2W362F-expressing cells are significantly (p < 0.04) different from those recorded in wild-type cells.

F1-160: Values in Kv1.5W461F-expressing cells are significantly (p < 0.001) different from those recorded in Kv4.2W362F-expressing cells.

In previous studies, we also demonstrated that expression of a pore mutant of Kv4.2, Kv4.2W362F, which functions as a Kv4-specific dominant negative (Barry et al., 1998), eliminatesI_Af in most (∼70%) SCG neurons (Malin and Nerbonne, 2000). The expression of Kv4.2W362F (Fig. 1,middle panel) results in a marked increase in type III cells, which express only I_K andI_SS, and reveals a novel phenotype (i.e., one not seen in wild-type SCG cells) in which onlyI_As,I_K, andI_SS are present (Malin and Nerbonne, 2000). In a subset (∼20%) of type I SCG cells expressing Kv4.2W362F (Fig. 1, middle panel, top), however,I_Af is evident (Table 1). These observations suggested either that Kv4.2W362F expression was insufficient to eliminate I_Af in some cells or, alternatively, that the fast transient outward K⁺ current in a subset of type I SCG neurons is not encoded by α-subunits of the Kv4 subfamily. The mean density of I_Af (64 ± 20 pA/pF) in type I cells expressing Kv4.2W362F is not significantly different from the mean I_Af density (81 ± 11 pA/pF) in wild-type I cells (Table 1). Analysis of the decay phases of the currents, however, revealed that the mean ± SEM inactivation time constant (190 ± 18 msec) of the residualI_Af is significantly larger than in wild-type (I or II) SCG cells (Table 1) (Malin and Nerbonne, 2000). In addition, examination of the distribution ofI_Af inactivation time constants (Fig.2) in wild-type I SCG cells suggested the presence of two distinct I_Afcomponents: one with τ_decay values between 50 and 130 msec, and another with τ_decay values between 131 and 210 msec (Fig. 2). Interestingly, expression of Kv4.2W362F dramatically shifts the distribution of τ_decay values in the remaining type I cells; the range of τ_decay values in Kv4.2W362F-expressing type I cells is 138–249 msec, with a mean of 190 ± 18 msec (Fig. 2) (Malin and Nerbonne, 2000). These observations were interpreted as suggesting that, in a subset of type I SCG cells, I_Af is not encoded by α-subunits of the Kv4 subfamily (Malin and Nerbonne, 2000).

Fig. 2.

Distinct components ofI_Af inactivation in SCG neurons. The time constants of inactivation of the rapid component of current decay,I_Af, were determined in wild-type, Kv4.2W362F-expressing, and Kv1.5W461F-expressing SCG cells as described in Materials and Methods. The τ_decay values were binned in 40 msec increments for comparison purposes and, as is evident, the distributions of τ_decay values in wild-type, Kv4.2W362F-expressing, and Kv1.5W461F-expressing SCG cells are distinct.

Expression of Kv1 α-subunits in SCG neurons

Although the previous studies clearly documented a role for Kv4 α-subunits in the generation of I_Afin the majority of SCG neurons (Malin and Nerbonne, 2000), the molecular correlate(s) of the residualI_Af, as well asI_As,I_K, andI_SS, remain to be identified. Previous molecular studies have demonstrated that several Kv α-subunits, in addition to Kv4.2 and Kv4.3, as well as several Kv β-subunits are expressed in rat SCG neurons (Dixon and McKinnon, 1996; Pankevych et al., 1999). In heterologous systems, expression of Kv1 α-subunits, alone or in combination with accessory β-subunits, gives rise to voltage-gated K⁺ currents with diverse kinetic and voltage-dependent properties, suggesting that Kv1 subfamily members may contribute to one or more outward K⁺ currents in rat SCG (and other) neurons. Importantly, previous studies have shown that several Kv1 α-subunits, including Kv1.1, Kv1.2, Kv1.4, and Kv1.5, and Kv β-subunits are expressed at the message level in SCG neurons (Dixon and McKinnon, 1996; Pankevych et al., 1999), suggesting that Kv1 α-subunits likely play a role in the generation of voltage-gated K⁺ currents in these cells. To examine Kv1 α-subunit protein expression in SCG cells, therefore, immunohistochemical experiments with anti-Kv1 α-subunit-specific antibodies were undertaken. These experiments revealed that Kv1 α-subunits are readily detected in isolated SCG neurons at the protein level (Fig. 3). As is illustrated in Figure 3B, for example, Kv1.4 expression is readily detected in the cell bodies of isolated SCG neurons. The expression pattern for Kv1.2 is distinct (from that of Kv1.4) in that Kv1.2 staining is seen throughout the processes of isolated SCG neurons, whereas Kv1.4 is only detected in cell bodies and proximal processes (Fig. 3A,B). In addition, all SCG cells are labeled with the anti-Kv1.2 antibody (Fig. 3A), whereas only a subset of cells express Kv1.4 (Fig. 3B). In contrast, Kv1.5 protein expression was not detected in SCG neurons (Fig. 3C).

Fig. 3.

Expression of Kv1 α-subunits in SCG neurons. Isolated wild-type SCG neurons were fixed and probed with polyclonal antibodies generated against Kv1.2 (A), Kv1.4 (B), or Kv1.5 (C) 48 hr after plating, as described in Materials and Methods. As is evident, the Kv1.2 and Kv1.4 α-subunits are readily detected in SCG neurons, although the staining patterns of these subunits are distinct. Scale bars, 50 μm.

Expression of Kv1.5W461F eliminates I_Afin a subset of SCG neurons

To explore directly the role of Kv1 α-subunits, the Kv1 subfamily-specific dominant negative construct, Kv1.5W461F-myc (Li et al., 1999), was used. Previous studies have shown that heterologous co-expression [in human embryonic kidney (HEK)-293 cells] of Kv1.5W461F-myc with either Kv1.5 or Kv1.4 attenuates current amplitudes relative to cells expressing (wild-type) Kv1.5 or Kv1.4 alone (Li et al., 1999). To examine the effects of Kv1.5W461F expression on the outward K⁺ currents in SCG neurons, beads were coated with cDNA constructs encoding Kv1.5W461F-myc and EGFP, and SCG cells were transfected using the Biolistic Gene Gun (see Materials and Methods). Within ∼24 hr of transfection, EGFP was readily detected under epifluorescence illumination (Fig.4); ∼10% of the cells in these cultures were EGFP-positive. To determine whether EGFP-positive SCG cells also express the Kv1.5W461F transgene, the cultures were fixed ∼48 hr after transfection and probed with an anti-mycantibody. These experiments revealed that all EGFP-positive cells (n = 100) also express Kv1.5W461F-myc. An example of an EGFP-positive, Kv1.5W461F-myc-positive SCG cell is shown in Figure 4, (bottom panels). Themyc staining appears to be predominantly on the cell surface (arrowheads), whereas EGFP expression is detected in the cytosol (Fig. 4, bottom).

Fig. 4.

Expression of Kv1.5W461F-myc in transfected SCG neurons. Isolated SCG neurons, transfected with EGFP alone (top) or with Kv1.5W461F-myc and EGFP (bottom) using the gene gun, were fixed and stained 24 hr later (see Materials and Methods). The top andbottom panels show EGFP fluorescence (left) and Cy3 fluorescence (right) images of the same field. Anti-myc staining is only evident in cultures transfected with Kv1.5W461F-myc (compare right panels,top and bottom). In addition, EGFP expression correlates with Kv1.5W461F (bottom, compare left and right panels). Scale bar, 50 μm.

Representative whole-cell voltage-gated outward K⁺ current waveforms recorded from Kv1.5W461F-expressing SCG cells are presented in Figure 1 (right panel). In Kv1.5W461F-expressing type I cells (Fig. 1,right panel), I_Af is evident, and the mean ± SEM current density (57 ± 6 pA/pF) is similar to that seen in wild-type I cells (Table 1). Mean ± SEM I_K (90 ± 13 pA/pF) andI_SS (52 ± 8 pA/pF) densities in Kv1.5W461F-expressing type I cells are also similar to the densities in wild-type I cells (Table 1). The percentage (44%; 15 of 34) of type I SCG neurons, however, is substantially lower than seen in recordings from wild-type cells, in which 70% of cells are classified as type I (Fig. 1, left panel). In parallel with the decrease in the percentage of type I cells, there is a marked increase in the percentage of type III cells (Fig. 1, compare left andright panels), which express onlyI_K andI_SS. In contrast, the percentage of Kv1.5W461F-expressing cells classified as type II is similar to the percentage of wild-type cells displaying this phenotype (Fig. 1, compare left and right panels). The densities of the currents and mean ± SEM τ_decay values for I_Af (72 ± 8 msec),I_As (585 ± 47 msec), andI_K (3125 ± 438 msec) in Kv1.5W461F-expressing type II cells are not significantly different from those in wild-type II cells (Table 1). These observations suggest that Kv1 α-subunits do not contribute measurably to the voltage-gated outward K⁺ currents in wild-type II SCG cells (see Discussion).

The concomitant decrease in type I cells and the increase in type III cells with the expression of Kv1.5W461F suggests that the functional removal of channels encoded by Kv1 α-subunits converts ∼25% of type I cells to type III by specific elimination ofI_Af. The expression of Kv1.5W461F also results in a shift in the distribution of τ_decay values forI_Af (Fig. 2). In cells expressing Kv1.5W461F, τ_decay values range from 54 to 150 msec, with a mean ± SEM value of 88 ± 8 msec (Table 1). This value is significantly (p < 0.001) smaller than the mean ± SEM τ_decay (190 ± 18 msec) for I_Af remaining in Kv4.2W362F-expressing cells (Table 1, Fig. 2), consistent with the hypothesis that there are two molecularly distinct components ofI_Af.

I_Af is eliminated in all SCG cells co-expressing Kv4.2W362F and Kv1.5W461F

To test the hypothesis that there are two populations ofI_Af channels encoded by distinct Kv subfamilies, SCG cells were cotransfected with Kv1.5W461F and Kv4.2W362F (and EGFP). Representative outward K⁺ currents recorded from EGFP-positive cells are presented in Figure 5. As is evident, the rapidly inactivating I_Af, prominent in wild-type SCG cells (Fig. 1, left panels), is not evident in cells expressing Kv4.2W362F and Kv1.5W461F (Fig. 5). The majority (∼75%) of Kv4.2W362F–Kv1.5W461F-expressing cells are classified as type III (Fig. 5). This is a significant increase in type III cells, compared with wild-type, Kv4.2W362F-expressing, or Kv1.5W461F-expressing cells (Fig. 1). The mean ± SEM densities ofI_K (135 ± 60 pA/pF) andI_SS (86 ± 12 pA/pF) in the Kv1.5W461F–Kv4.2W362F-expressing type III cells are not significantly different from the densities of these currents determined in wild-type III cells (Table 1). Analysis of the outward K⁺ currents in the remaining (∼25%) cells reveals the presence of I_As,I_K, andI_SS, suggesting that these are type II cells lacking I_Af (Table 1).

Fig. 5.

Coexpression of both Kv1.5W461F and Kv4.2W362F eliminates I_Af. Isolated SCG neurons were transfected with Kv1.5W461F, Kv4.2W362F, and EGFP using the Biolistic Gene Gun (as described in Materials and Methods), and outward K⁺ currents were recorded from EGFP-positive cells as described in the legend to Figure 1. Two distinct current waveforms were evident in these recordings: the vast majority (76%) of cells were found to express only I_K andI_SS and were classified as type III (B); the remaining cells (24%) expressI_As,I_K, andI_SS, and, therefore, are type II cells lacking I_Af (A). The fast transient current I_Af was not detected in any of these cells (n = 15). Analysis of the decay phases of the currents revealed that the densities ofI_As,I_K, andI_SS in Kv1.5W461F + Kv4.2W362F-expressing type II and III cells are indistinguishable from the currents in wild-type II and III cells (Table 1).

Expression of Kv1.5W461F increases excitability of SCG cells

Subsequent experiments were aimed at determining the effects of eliminating the Kv1-mediated I_Af on the waveforms of individual action potentials and the repetitive firing properties of SCG neurons. As reported previously, current-clamp recordings from wild-type SCG cells reveal that ∼45% of the cells are “phasic,” firing one or two action potentials in response to a prolonged (500 msec) depolarizing current injection (Malin and Nerbonne, 2000). Increasing the amplitude of the injected current does not markedly influence the number of action potentials recorded (Malin and Nerbonne, 2000). Approximately 30% of wild-type SCG cells display the “adapting” phenotype. In these cells, low-amplitude current injections produce repetitive firing, and the firing frequency decreases over time. At higher amplitude current injections, adapting cells cease firing and are refractory (Malin and Nerbonne, 2000). Adapting cells are further distinguished from phasic cells by increased input resistances and decreased current thresholds for action potential generation (Table 2). The remaining (∼25%) wild-type SCG neurons are “tonic,” regular-spiking cells that fire at a fixed frequency during prolonged current injections up to 300 pA (Malin and Nerbonne, 2000). In addition, individual action potentials in tonic cells are brief (Table 2), and the firing frequency increases with the amplitude of the injected current (Malin and Nerbonne, 2000). Briefer action potential durations and the lack of adaptation (Table 2) clearly distinguish tonic from phasic and adapting SCG neurons.

Table 2.

Effects of Kv1.5W461F and Kv4.2W362F expression on SCG neuron firing properties ^2-a

		Rin(GΩ)	Vm (mV)	APThresh(pA)	APA (mV)	APD50 (msec)	APD90(msec)	n
Wild type	All cells	0.34  ± 0.03	−48 ± 1	46  ± 5	82  ± 4	3.66  ± 0.14	5.92  ± 0.20	28
	Phasic	0.26  ± 0.02	−45 ± 2	67  ± 1	80  ± 5	4.18  ± 0.24	6.55  ± 0.34	12
	Adapting	0.60  ± 0.06	−48 ± 1	24  ± 2	87  ± 3	3.57  ± 0.20	5.96  ± 0.20	9
	Tonic	0.22  ± 0.02	−52 ± 1	42  ± 2	91  ± 3	2.97  ± 0.03	4.87  ± 0.20	7
Kv1.5W461F	All cells	0.38  ± 0.04	−48 ± 1	32  ± 3	89  ± 8	3.08  ± 0.14	5.26  ± 0.25	25
	Phasic	0.30  ± 0.03	−49 ± 2	43  ± 62-160	90  ± 3*	3.16  ± 0.18*	5.36  ± 0.37*	6
	Adapting	0.48  ± 0.06	−47 ± 2	24  ± 2	89  ± 3	3.25  ± 0.24	5.51  ± 0.45	11
	Tonic	0.30  ± 0.05	−50 ± 1	36  ± 5	92  ± 2	2.77  ± 0.21	4.84  ± 0.41	8
Kv4.2W362F	All cells	0.68  ± 0.08	−49 ± 1	28  ± 2	90  ± 2	3.54  ± 0.27	5.59  ± 0.22	25
	Phasic	0.64  ± 0.142-160	−47 ± 2	30  ± 72-160	82  ± 3	3.63  ± 0.44	6.07  ± 0.33	6
	Adapting	0.76  ± 0.12	−47 ± 1	26  ± 3	89  ± 2	3.47  ± 0.14	5.76  ± 0.23	13
	Tonic	0.60  ± 0.092-160	−53 ± 3	27  ± 22-160	99  ± 4	2.90  ± 0.09	4.90  ± 0.20	6
Kv1.5W461F + Kv4.2W362F	All cells	1.08  ± 0.172-160	−44 ± 2	35  ± 4	88  ± 3	3.07  ± 0.19*	5.11  ± 0.30*	38
	Phasic	0.36  ± 0.04*	−43 ± 4	38  ± 72-160	90  ± 7	2.76  ± 0.242-160	4.71  ± 0.312-160	5
	Adapting	1.28  ± 0.17*	−42 ± 2	25  ± 2	81  ± 6	3.75  ± 0.43	6.11  ± 0.66	17
	Tonic	0.47  ± 0.042-160	−47 ± 2	31  ± 5	94  ± 4	2.65  ± 0.24	4.54  ± 0.39	16

^F2-aAll values are means ± SEM. R_in, Input resistance;V_m, resting membrane potential; AP_thresh, current required to reach the threshold for generating an action potential; APA, action potential amplitude.

Mean values are significantly different from those in wild-type cells at the

^*p < 0.03 and

F2-160: p < 0.005 levels.

The phasic, adapting, and tonic firing patterns are also seen in recordings obtained from Kv1.5W461F-expressing SCG cells (Fig.6). The distribution of SCG cell firing patterns, however, is markedly affected by Kv1.5W461F expression. Functional elimination of Kv1-encodedI_Af channels reduces the number of phasic cells (Fig. 6A) from 43% of wild-type to 24% of Kv1.5W461F-expressing cells (Table 2). In contrast, the percentage of adapting cells (Fig. 6B) increases from 32% of wild-type to 44% of Kv1.5W461F-expressing cells (Table 2). The percentage of tonic cells (Fig. 6C) is also increased slightly (from 25 to 32%) with Kv1.5W461F expression (Table 2). Thus, the selective removal of the Kv1-mediatedI_Af converts many (∼40%) phasic cells to the adapting or tonic firing patterns (Fig. 6, Table 2). Interestingly, the percentage of type I cells with Kv1-encodedI_Af channels (∼25%) is very similar to the percentage of cells (∼20%) converted from phasic firing with Kv1.5W461F expression, suggesting that type I cells with Kv1-encodedI_Af channels are phasic (see Discussion). In contrast to the findings with Kv4.2W362F, however, expression of Kv1.5W461F does not affect the input resistances of phasic or tonic cells (Table 2). Thus, the properties of Kv1- and Kv4-encoded I_Af channels are distinct, in that Kv1-encoded I_Af channels are not open at rest and, therefore, do not contribute to the resting input resistances of SCG neurons.

Fig. 6.

Expression of Kv1.5W461F increases the percentage of adapting cells. Isolated SCG neurons were transfected with Kv1.5W461F (and EGFP), and action potentials and repetitive firing patterns were recorded in response to brief or prolonged depolarizing current injections, as described in Materials and Methods. Current-clamp recordings from (3) representative Kv1.5W461F-expressing cells are shown in A, B, andC. In each cell, single action potentials were elicited by 1.5 msec current injections (left), and repetitive firing patterns were recorded in response to 100 pA (middle) or 200 pA (right) 500 msec current injections. On the basis of the response(s) to the 500 msec current injections, cells were classified as phasic (A), adapting (B), or tonic (C) (Table 2). With Kv1.5W461F expression, the percentages of adapting and tonic cells are increased, and the percentage of phasic cells is decreased relative to the firing pattern distribution seen in wild-type cells (Table 2).

Analysis of the properties of the phasic firing cells remaining with Kv1.5W461F expression revealed that the mean ± SEM current threshold for action potential generation in the phasic cells lacking Kv1-encoded I_Af (i.e., the remaining phasic cells) is significantly (p < 0.005) lower than the mean determined in wild-type phasic cells (Table 2). In addition, the mean ± SEM action potential amplitude in the remaining phasic cells is significantly (p < 0.03) larger than in wild-type phasic cells (Table 2). These observations are consistent with the suggestion that there are two distinct populations of phasic SCG cells and, further, that the current thresholds for action potential generation are lower and action potential amplitudes are higher in the subset of phasic cells lacking Kv1-encoded I_Af channels. Action potential durations in the remaining phasic cells are also decreased significantly (p < 0.005) compared with wild-type phasic cells (Table 2). This unexpected observation suggests that there are additional repolarizing currents activated in phasic cells lacking Kv1-encoded I_Afchannels, perhaps because of the increased action potential amplitudes in these cells (Table 2). The mean ± SEM current threshold values for action potential generation, as well as action potential amplitudes and durations, in adapting and tonic SCG cells, in contrast, are not affected by Kv1.5W461F expression (Table 2), consistent with the suggestion that Kv1-encoded I_Afchannels specifically tune excitability in phasic cells (see Discussion).

Complete removal of I_Af markedly increases excitability and further reduces the percentage of phasic cells

In SCG neurons transfected with both the Kv1.5W461F and the Kv4.2W362F dominant negative constructs, the distribution of repetitive firing patterns is altered markedly (Fig.7D) compared with wild-type cells (Fig. 7A), as well as with cells expressing Kv4.2W362F (Fig. 7B) or Kv1.5W461F (Fig. 7C) alone. After the complete removal of I_Af, few phasic cells remain (13%) (Fig. 7D), and the percentages of adapting and tonic cells are increased. These effects appear to be the sum of the Kv1- and Kv4-mediated changes; removal of either the Kv1- or the Kv4-encoded I_Af channels alone increases the percentage of adapting cells at the expense of phasic cells, and removal of Kv1 channels also increases tonic cell number (Fig. 7). As seen with the expression of Kv4.2W362F alone, the mean ± SEM input resistance of SCG cells expressing Kv1.5W461F and Kv4.2W362F is significantly (p < 0.005) increased compared with wild-type cells (Table 2). These findings are expected because expression of Kv4.2W362F alone significantly affects the mean ± SEM input resistance (Table 2). Expression of Kv1.5W461F and Kv4.2W362F, like expression of either Kv1.5W461F or Kv4.2W362F alone, also decreases the current thresholds for action potential generation (Table 2).

Fig. 7.

Elimination of the Kv1- and Kv4-encodedI_Af increases the percentage of adapting SCG neurons. Action potentials and repetitive firing patterns were recorded from isolated SCG neurons 24 hr after transfection with EGFP alone (A), EGFP and Kv4.2W362F (B), EGFP and Kv1.5W461F (C), or EGFP, Kv1.5W461F and Kv4.2W362F (D), as described in the legend to Figure 6. The phasic (left), adapting (middle), and tonic (right) firing patterns were evident in recordings obtained under all of these experimental conditions. In recordings from cells with reduced I_Af density, the percentages of phasic cells are lower, and the percentages of adapting and tonic cells are higher than seen in recordings from wild-type cells (Tables 2, 4, 5).

Increasing Kv1-mediated I_Af converts adapting cells to phasic firing

The experiments described above demonstrate a role for Kv1 α-subunits in the generation of I_Afin a subset of type I phasic-firing SCG neurons. Of the Kv1 α-subunits, only Kv1.4 produces rapidly inactivating outward K⁺ currents when expressed alone in heterologous expression systems (Coetzee et al., 1999). In addition, Kv1.4 has recently been shown to underlie the slow transient outward K⁺ current in mammalian cardiac cells (Guo et al., 1999, 2000), and Kv1.4 is expressed at the protein level in SCG neurons (Fig. 3). Subsequent experiments, therefore, were aimed at examining the effects of “over-expression” of Kv1.4 on the outward K⁺ currents and the firing properties of SCG neurons. Representative current-clamp recordings from Kv1.4-expressing cells are shown in Figure8. The percentage of phasic cells (Fig.8A) is increased markedly (to 67%) with Kv1.4 expression, relative to the percentage (43%) of wild-type phasic SCG cells (Table 3). The fraction of adapting cells is decreased (Fig. 8B), whereas the percentage of tonic cells is unchanged (Fig. 8C, Table 3). Indeed, of the 18 cells examined, only two adapting cells were detected, suggesting that Kv1.4 expression converts most adapting cells to phasic firing. Consistent with the increase in phasic cells and the decrease in adapting cells, the mean ± SEM current threshold for action potential generation is increased significantly (p < 0.005) in all Kv1.4-expressing SCG cells (Table 3). These data support our hypothesis that the current threshold for action potential generation is determined largely byI_Af density and is linked to firing pattern. Expression of Kv1.4 also significantly (p < 0.04) decreases mean ± SEM APD₅₀ values in phasic cells, whereas action potential durations in Kv1.4-expressing tonic and adapting cells are not significantly different from those in wild-type tonic and adapting cells (Table 3).

Fig. 8.

Expression of Kv1.4 increases the percentage of phasic firing SCG cells. Action potentials and repetitive firing patterns, recorded as described in the legend to Figure 6, were obtained from isolated SCG neurons 24 hr after transfection with wild-type Kv1.4 and EGFP. As in wild-type cells, the phasic (A), adapting (B), and tonic (C) firing patterns were seen in recordings from cells transfected with Kv1.4. The percentage of adapting cells, however, is lower and the percentage of phasic cells is higher in Kv1.4-expressing cells than seen in recordings from wild-type or Kv1.5W461F-expressing SCG cells (Figs. 6, 7, Tables 2, 4).

Table 3.

Expression of Kv1.4 decreases the excitability of SCG neurons ^3-a

	Rin (GΩ)	Vm(mV)	APThresh (pA)	APA (mV)	APD50(msec)	APD90 (msec)	n
All cells	0.40  ± 0.09	−47  ± 2	80  ± 123-150	84  ± 3	3.24  ± 0.23	5.50  ± 0.36	18
Phasic	0.39  ± 0.12	−46  ± 3	100  ± 153-160	79  ± 3	3.29  ± 0.323-160	5.53  ± 0.53	12
Adapting	0.71	−47	20	91	3.72	6.15	2
Tonic	0.29  ± 0.05	−52  ± 2	50  ± 9	95  ± 3	2.86  ± 0.08	5.10  ± 0.14	4

^F3-aAll values are means ± SEM.

Values are significantly different from those in wild-type cells at the

F3-150: p < 0.005 and

F3-160: p< 0.04 levels (Table 2).

Representative voltage-clamp recordings obtained from SCG neurons expressing Kv1.4 (and EGFP) are shown in Figure9. Analysis of the decay phases of the currents revealed that I_Af density is increased significantly (p < 0.02) to a mean ± SEM value of 141 ± 9 pA/pF in Kv1.4-expressing type I cells. In addition, the mean ± SEM τ_decay value forI_Af (169 ± 10 msec) in Kv1.4-expressing type I cells is significantly larger than the mean time constant for I_Af (121 ± 14 msec) in wild-type I cells (Table 1). Interestingly, over-expression of Kv4.2 reduces the mean ± SEM τ_decay forI_Af (Table 3), revealing that Kv1.4 and Kv4.2 encode kinetically distinct K⁺currents when expressed in sympathetic neurons. These differences are also reflected in the ranges of I_Afτ_decay values seen. In wild-type I cells, for example, I_Afτ_decay values range from 40 to 209 msec (Fig.9D), whereas in SCG cells expressing Kv1.4,I_Af τ_decayvalues range from 142 to 205 msec. This change is distinct from that seen in SCG cells expressing Kv4.2, in which τ_decay values range from 50 to 128 msec (Fig.9D, Table 4). Thus, exogenous Kv1.4 encodes a fast transient current in SCG neurons with inactivation kinetics similar to the component eliminated with Kv1.5W461F expression (Fig. 1, Tables 1, 4). Furthermore, this component is kinetically distinct from that encoded by Kv4.2 (Fig. 9D, Table 4).

Fig. 9.

Expression of Kv1.4 increasesI_Af only in type I SCG neurons. Isolated SCG neurons were transfected with Kv1.4 and EGFP using the Biolistic Gene Gun (as described in Materials and Methods), and outward K⁺ currents were recorded from EGFP-expressing cells as described in the legend to Figure 1. Analysis of the decay phases of the currents provided the mean ± SEM densities ofI_Af,I_As,I_K, andI_SS (Table 1) and allowed classification of Kv1.4-expressing cells as type I (A), type II (B), or type III (C). The distribution of (type I, type II, and type III) cells is unaffected by Kv1.4 expression, and the mean ± SEMI_Af density is increased significantly (p < 0.001) in type I SCG cells, as compared with wild-type I cells (Table 1). Interestingly, type II and type III cells are unaffected by expression of Kv1.4. The distributions of I_Af τ_decay values in wild-type, Kv4.2-expressing, and Kv1.4-expressing type I cells are distinct, consistent with the hypothesis that Kv4.2 and Kv1.4 encode kinetically distinct fast transient currents in SCG cells (D).

Table 4.

SCG neurons transfected with wild-type Kv4.2 or Kv1.4 ^4-a

	Kv4.24-b		Kv1.4
	Type I	Type II	Type I	Type II	Type III
Peak current density	249  ± 27	291  ± 37	281  ± 39	275  ± 34	156  ± 45
IAf
Density (pA/pF)	127  ± 94-150	136  ± 16	141  ± 94-150	83  ± 8
τ (msec)	87  ± 10	70  ± 8	169  ± 104-160	66  ± 10
IAs
Density (pA/pF)		57  ± 21		30  ± 7
τ (msec)		472  ± 8		557  ± 107
IK
Density (pA/pF)	78  ± 17	67  ± 2	78  ± 14	101  ± 31	119  ± 36
τ (msec)	2126  ± 518	2151  ± 195	2050  ± 261	3588  ± 344	4031  ± 466
ISS
Density (pA/pF)	40  ± 6	38  ± 17	54  ± 12	74  ± 9	43  ± 13
n	11	5	7	3	2

^F4-aAll values are mean ± SEM; current densities and τ_decay values were determined for currents evoked at +50 mV; n = number of cells.

^F4-bData from Malin and Nerbonne, (2000).

F4-150: Values are significantly (p < 0.02) higher than in wild-type I SCG cells (see Table 1);

F4-160: values are significantly (p < 0.001) larger than in Kv4.2-transfected cells.

In contrast, mean I_K andI_SS densities, as well as the kinetics of I_K decay, are not significantly different in Kv1.4-expressing and wild-type I cells (compare Tables 1,4). Interestingly, Kv1.4 expression has no effect onI_Af density (83 ± 8 pA/pF) in type II cells (Fig. 9B, Table 4). The other currents,I_As,I_K, andI_SS, in type II cells are also unaffected by Kv1.4 expression (Table 4). Similarly, the densities and properties of I_K andI_SS in type III cells expressing Kv1.4 are indistinguishable from those seen in wild-type III cells (Tables 1,4). Together, these results suggest that functional Kv1-encodedI_Af channels are not expressed in type II or type III cells even when the (wild-type) Kv1.4 α-subunit is exogenously introduced (see Discussion).

DISCUSSION

Kv1 α-subunits underlie I_Af in a subset of type I SCG neurons

The results of the experiments reported here demonstrate that expression of Kv1.5W461F results in the elimination ofI_Af in a subset of type I SCG cells and a corresponding increase in the percentage of type III cells (Fig.1, Table 1). The properties and the densities of the currents in Kv1.5W461F-expressing type II and type III cells are indistinguishable from the currents in wild-type II and III cells, suggesting that Kv1 α-subunits do not make a significant contribution toI_Af in type II cells or toI_K andI_SS in type II and III cells. Importantly, the experiments here also demonstrate that the residualI_Af present in Kv4.2W362F-expressing type I SCG cells (Malin and Nerbonne, 2000) does not reflect incomplete suppression of Kv4-encoded I_Afchannels. Rather, the residual I_Af in the Kv4.2W362F-expressing SCG cells reflects Kv1-encodedI_Af channels. In addition, the mean ± SEM I_Afτ_decay values determined in Kv1.5W461F- and Kv4.2W362F-expressing cells are significantly (p< 0.001) different (Fig. 2), consistent with the hypothesis that there are two types of I_Af channels in wild-type SCG neurons that reflect the expression of distinct gene products. Because co-expression of Kv1.5W461F and Kv4.2W362F eliminatesI_Af in all SCG cells (Fig. 5, Table1), it is also possible to conclude that Kv1 and Kv4 α-subunits must encode all of the fast transient current (I_Af) in these cells.

Previous studies have demonstrated the presence of distinct types of transient outward K⁺ currents in neurons and muscle cells and shown that these are encoded by Kv1 and Kv4 α-subunits (Solc et al., 1987; Tsunoda and Salkoff, 1995; Guo et al., 1999, 2000). In contrast to the findings here, however, the transient outward K⁺ currents inDrosophila neurons and myocytes (Solc et al., 1987; Tsunoda and Salkoff, 1995), as well as those in mammalian cardiac myocytes (Guo et al., 1999, 2000), display markedly different time- and voltage-dependent properties and are readily distinguished electrophysiologically. Interestingly, however, the identification of two molecularly distinct components of a “single” electrophysiologically defined current has been reported previously forI_Kr in Xenopus spinal neurons (Ribera, 1996) and for I_{K, slow} in mouse ventricular myocytes (Xu et al., 1999). These findings corroborate the power of the molecular pharmacological approach exploited here and in these previous studies (Ribera, 1996; Xu et al., 1999). The Kv α-subunit-specific dominant negative strategy selectively eliminates individual current components, allowing the characterization of detailed properties of the currents remaining (as well as of the currents eliminated). In the case ofI_Af in SCG neurons, experiments completed on cells expressing Kv1.5W461F or Kv4.2W362F revealed the presence of two current components that had not previously been distinguished using conventional electrophysiological and/or pharmacological methods. The molecular pharmacological approach exploited here also allowed determination of the functional roles of the individual Kv1- and Kv4-encoded components ofI_Af in shaping the waveforms of individual action potentials and repetitive firing patterns in SCG neurons.

Functional roles of I_Af in SCG neurons

The experiments completed here reveal that the functional consequences of eliminating the Kv1- or the Kv4-encodedI_Af channels in SCG cells are distinct, suggesting that the molecular heterogeneity inI_Af has physiological significance. Elimination of Kv1-encoded I_Afincreases excitability by decreasing the current threshold for action potential generation (Table 5), without measurably affecting cell input resistances. Removal of Kv4-encodedI_Af channels also increases excitability by lowering current thresholds, although, in this case, input resistances are also increased (Table 5) (Malin and Nerbonne, 2000). In addition, the Kv1- and Kv4-encodedI_Af channels play different roles in controlling repetitive firing in SCG neurons. Although elimination of either Kv1- or Kv4-encoded I_Af reduces (by ∼50%) the number of phasic cells (Table 5), removal of the Kv4-encoded I_Af converts phasic cells to adapting firing, whereas removal of the Kv1-encodedI_Af converts some phasic cells to adapting and others to tonic firing (Fig. 7, Table 2). Interestingly, co-expression of Kv1.5W461F and Kv4.2W362F dramatically reduces, but does not eliminate, the number (percentage) of phasic cells. This finding suggests that, although I_Af is an important contributor, it is not the sole determinant of phasic firing. In addition, expression of Kv4.2W362F significantly (p < 0.02) increases the mean (±SEM) firing rates in tonic cells (in response to 200 pA current injections) to 29 ± 2 Hz from the wild-type value of 15 ± 1 Hz. There is also an increase in the mean ± SEM tonic firing rate in Kv1.5W461F-expressing cells, although, in this case, the increase (to 20 ± 6 Hz) is modest (∼30%) and most Kv1.5W461F-expressing tonic cells fire at frequencies well within the wild-type range (Fig.7).

Table 5.

Phenotypic consequences of eliminating the Kv1- or Kv4-encoded components of I_Af

indicates changes common to Kv1- and Kv4-encoded I_Afelimination.

As noted above, the experiments here revealed that the voltage-clamp effects of Kv1.5W461F expression are specific to type I cells; expression of Kv1.5W461F removes I_Afin ∼25% of SCG cells, all of which are type I (Fig. 1, Table 1). In addition, expression of Kv1.5W461F converts ∼50% of phasic firing rat SCG cells to the adapting or tonic firing patterns (Fig. 7, Table5). Thus, we can conclude that ∼50% of the phasic cells are type I. The experiments here also revealed that the mean (±SEM) active and passive membrane properties of the phasic cells remaining with Kv1.5W461F expression are distinct from those of wild-type phasic cells (Table 3). Specifically, action potential amplitudes are greater, and action potential durations are shorter in the phasic cells lacking Kv1-encoded I_Af channels (i.e., the phasic cells remaining with Kv1.5W461F expression). In contrast, action potential amplitudes and durations in Kv4.2W362F-expressing and wild-type phasic cells are indistinguishable (Table 5). These observations suggest that in the phasic cells expressing Kv1-encodedI_Af channels, action potential amplitudes are lower, and action potential durations are longer than in phasic cells expressing Kv4-encodedI_Af channels.

The distinct functional roles of Kv1- and Kv4-encodedI_Af likely reflect differences in the voltage dependences of activation of the currents. The results of experiments completed here suggest, for example, that the Kv4-encodedI_Af channels are open at more hyperpolarized potentials (than the Kv1-encoded channels) and affect neuronal excitability by decreasing resting SCG cell input resistance (Malin and Nerbonne, 2000). The Kv1-encodedI_Af channels, in contrast, likely are only activated at more depolarized potentials and influence excitability, therefore, by opposing the action potential upstroke; Kv1-encoded I_Af channels do not contribute to resting input resistance in SCG cells. In addition to this functional diversity, the expression of molecularly distinctI_Af channels encoded by Kv1 and Kv4 α-subunits also provides a means for differential modulation of channel, and therefore, cellular, functioning through interactions with distinct transmitters and/or intracellular messenger pathways (Roeper et al., 1997; Wang et al., 1997; Pan et al., 2000). Clearly, experiments aimed at exploring the molecular mechanisms involved in the regulation and modulation of Kv1- and Kv4-encoded neuronalI_Af channels will be of considerable interest.