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. 2019 Sep 23;8:e48056. doi: 10.7554/eLife.48056

Figure 1. Mamo expression coincides with the generation of α’/β’ neurons in the Mushroom Body (MB) neuronal lineages.

(A) Temporal gradients specify postembryonic neurons of the MB lineages into three sequential neuronal classes (Lee et al., 1999; Liu et al., 2015). Newborn neurons are colored to illustrate expression levels of Imp (red), Syp (blue), and Chinmo (gray stars). ALH = after larval hatching, APF = after pupal formation. (B–D) MB lineages (OK107 > GFP) immunostained for GFP, Chinmo (Rat-anti-Chinmo), and Mamo at different developmental times. A single focal plane near the MB NB is shown. Newborn neurons (NN) are identified by the very dim GFP expression near the NB as described by Zhu et al. (2006) and outlined in white. Young/maturing neurons are immediately adjacent to the NNs with a slightly higher GFP intensity and outlined in yellow. Chinmo levels in NNs decline over time. Mamo staining is visible at 84 hr ALH in young/maturing neurons (C). At 24 hr APF, Mamo expression is strong in older neurons (gray dashed outline), but absent from young/maturing neurons (D). Scale bar = 20 μm. Images are representative of n > 18. The quantification of Chinmo and Mamo staining is in Figure 1—figure supplement 1.

Figure 1—source data 1. Intensity of Chinmo and Mamo staining at different developmental times.
elife-48056-fig1-data1.xlsx (11.5KB, xlsx)
DOI: 10.7554/eLife.48056.004

Figure 1.

Figure 1—figure supplement 1. Mamo expression coincides with the generation of α’/β’ neurons in the Mushroom Body (MB) neuronal lineages.

Figure 1—figure supplement 1.

(A) The intensity of Chinmo (gray line) and Mamo (Green line) antibody staining in MB neurons at different developmental times. The Chinmo levels were measured in the newborn neurons and Mamo levels were measured in the young/maturing neurons (mean ± SEM, n = 6 brains). AU, arbitrary fluorescent intensity units. (B–C) Mushroom body lineages (201y > GFP) immunostained for GFP (green), and Mamo (gray/magenta) at 84 hr ALH (B) and 0 hr APF (C). A single focal plane near the MB NB is shown. Mamo (white arrows) staining is visible at 84 hr ALH (B). However, Mamo staining with GFP-positive neurons is evident (MB γ neuron types, red arrow) at 0 hr APF (C). Note that white arrow indicates Mamo staining is not in the GFP-positive neurons. Scale bar = 20 μm. Images are representative of n > 6.