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. 2019 Sep 23;8:e48056. doi: 10.7554/eLife.48056

Figure 2. Weak Chinmo initiates Mamo protein expression.

MB lineages (OK107 > GFP) with different genetic manipulations immunostained for GFP and Mamo. A single focal plane near the MB NB is shown. Newborn neurons (NN) are outlined in white. Young/maturing neurons are outlined in yellow. Images are representative of n > 18. Scale bar = 20 μm. The diagram below shows approximate levels of Imp (red), Syp (blue), and Chinmo (stars) expressed in the young/maturing neurons when they were NNs ~ 12 hr prior (as reported by Liu et al., 2015), or Figure 3—figure supplement 1). (A-F) At 84 hr ALH, Mamo staining is visible only in the young/maturing neurons in control brains (A) and brains expressing chinmo-RNAi (D). (C) chinmo-/-; OK107 > GFP is a chinmo null MARCM clone induced at newly hatched larvae (NHL). Note that OK107 drives GFP only within the clone. MB neurons outside of the chinmo-/- clone (eyeless+, data not shown) express Mamo (yellow arrow). (F) At 60 hr ALH, Mamo staining is only visible after OK107 > chinmo RNAi. (G) Mean number (± SEM) of Mamo positive neurons per brain hemisphere in control (gray) and chinmo-RNAi (black) expressing MBs (***p<0.005, n = 4–5). The quantification of Mamo staining is in Figure 2—figure supplement 1.

Figure 2—source data 1. Quantification of Mamo positive neurons.
elife-48056-fig2-data1.xlsx (10KB, xlsx)
DOI: 10.7554/eLife.48056.007
Figure 2—source data 2. Mamo staining intensity in young/maturing neurons with chinmo manipulations.
elife-48056-fig2-data2.xlsx (11.4KB, xlsx)
DOI: 10.7554/eLife.48056.008

Figure 2.

Figure 2—figure supplement 1. The intensity quantification of Mamo staining in different manipulations of MB neurons.

Figure 2—figure supplement 1.

(A–B) The intensity of Mamo (Green line) staining in MB neurons at 84 hr ALH (A) and 60 hr ALH (B). The Mamo levels were measured in the young/maturing neurons (mean ± SEM, n = 5–6 brains). AU, arbitrary fluorescent intensity units.