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. 2019 Sep 18;24(18):3390. doi: 10.3390/molecules24183390

Figure 5.

Figure 5

Effects of trilobatin on the expression of HBXIP and HNF4α in HepG2 cells. (A) After HepG2 cells were treated with indicated dose of trilobatin for 24 h, the mRNA of HBXIP and HNF4α were measured by RT-PCR, Data are mean ± SD (n = 3). (B) After HepG2 cells were incubated with indicated concentrations of trilobatin for 24 h, the protein levels of HBXIP and HNF4α were detected with western blot. (C) After HepG2 cells were incubated with indicated concentrations of trilobatin, canagliflozin, empagliflozin and phloridzin for 24 h, the cell lysates were used to determine the protein levels of HBXIP and HNF4α by western blot.