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. 2019 Mar 23;8(1):83–97. doi: 10.1080/21623945.2019.1580842

Figure 8.

Figure 8.

Caco-2/HT29-MTX intestinal barriers pre-treated with oleic acid (OA) in co-culture induce TG accumulation in AML12 hepatocytes. (a) Intestinal barriers were pre-treated with either 10 or 20 μM OA during 2 h then OA was removed by replacing media and intestinal barrier 96-wells inserts were loaded onto AML12 hepatocytes during 24 h in HG media. TG accumulation was assessed using AdipoRed fluorescent intensities normalized to time of pre-treated intestinal barrier before loading onto AML12 cells in either Caco-2/HT29-MTX (T0) or 24 h after co-culture (A) and AML12 cells. Corresponding images are presented as merged AdipoRed (green)/Hoechst 33258 (red) images at magnification x4 (96-wells inserts) or x20 (AML12). Dot line at 1 represents the value for control at T = 0. (b) AML12 sensing of intestinal barriers pre-treated with OA (10 µM) in co-culture media containing either low (LG) or high glucose (HG) with or without insulin (INS 0,05 mU/mL) after 24 h. Data are presented as mean values ± SEM (n = 8) with significant Student t-test p-values <0.05 compared to T0.