Table 1.
Antibodies used in this study in alphabetical order.
| Antibody | Cat. No. | Clonality | Supplier/Reference | Species * | Dilution |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| α-smooth muscle actin (α-SMA) (ASM-1) | CBL-171 | mono (m) | Millipore, Merck, Darmstadt, Germany | h, m, r, b, ch, eq | 1:2000 |
| β-actin | A5441 | mono (m) | Sigma, Taufkirchen, Germany | h, m, r, gp, can, hm, f, p, car, c, rb, sh, b | 1:10,000 |
| Alix (1A12) | sc-43540 | mono (m) | Santa Cruz, Santa Cruz, CA, USA | h, m, r | 1:1000 |
| ALK5 (V-22) | sc-398 | poly (rb) | Santa Cruz | h, m, r | 1:500 |
| Caveolin-1 | CS-3238 | poly (rb) | Cell Signaling Technology, Frankfurt am Main, Germany | h, m, r, ha, z, b, p | 1:1000 |
| CD81 (B-11) | sc-166029 | mono (m) | Santa Cruz | h, m, r | 1:1000 |
| CTGF | sc-14939 | poly (g) | Santa Cruz | h, m, r | 1:1000 |
| Endoglin | AF1320 | poly (g) | R&D Systems, Wiesbaden, Germany | m | 1:2000 |
| MMP-14 | AB38971 | poly (rb) | Abcam, Cambridge, UK | h, m, p | 1:1000 |
| PPabE2 | NA | poly (rb) | [19] | r | 1:2000 |
| pSmad2 | CS-3101 | poly (rb) | Cell Signaling Technology | h, m, r | 1:1000 |
| TGF-β1 | CS-3711 | poly (rb) | Cell Signaling Technology | h, m, r | 1:500 |
| TGF-βRII (L-21) | sc-400 | poly (rb) | Santa Cruz | h, m, r | 1:500 |
| TGF-βRIII | AF242PB | poly (g) | R&D Systems | h | 1:1000 |
| Secondary antibodies | |||||
| IgG-HRP | sc-2004 | NA | Santa Cruz | rb | 1:5000 |
| IgG-HRP | sc-2005 | NA | Santa Cruz | m | 1:5000 |
| IgG-HRP | sc-2056 | NA | Santa Cruz | g | 1:5000 |
* Abbreviations used are: α-SMA—α-smooth muscle actin; a—avian; b—bovine; c—chicken; can—canine; car—carp; CTGF—connective tissue growth factor; eq—equine; f—feline; g—goat; gp—guinea pig; h—human; ha—hamster; hm—Hirudo medicinalis, m—mouse; mi—mink; mk—monkey; mono—monoclonal antibody; p—pig; poly—polyclonal antibody; NA—not applicable; o—ovine; p—pig; rb—rabbit; sh—sheep; z—zebrafish.