Table 1.
List of primary and secondary antibodies used in this study.
| Antigen | Species | Dilution | Supplier | |
|---|---|---|---|---|
| Primary | Brn3a | Goat | 1:250 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| GAP43 | Mouse | 1:500 | Invitrogen. Paisley, UK | |
| βIII-tubulin | Mouse | 1:200 | Sigma, Poole, UK | |
| GFAP | Mouse | 1:200 (ICC),1:250 (IHC) | Sigma, Poole, UK | |
| Phospho-S6 | Rabbit | 1:200 (ICC) | Cell Signalling Technology, Hitchin, UK | |
| RTP801 | Rabbit | 1:100 (ICC & IHC) | Abcam, Cambridge, UK | |
| GSK3β | Mouse | 1:200 (ICC & IHC) | Abcam, Cambridge, UK | |
| Secondary | Goat IgG AlexaFluor 488 | Donkey | 1:500 (IHC) | Invitrogen. Paisley, UK |
| Mouse IgG AlexaFluor 488 | Goat | 1:400 (ICC), 1:500 (IHC) | Invitrogen. Paisley, UK | |
| Rabbit IgG AlexaFluor 594 | Goat | 1:400 (ICC), 1:500 (IHC) | Invitrogen. Paisley, UK |