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. 2019 Sep 17;8(9):1096. doi: 10.3390/cells8091096

Figure 2.

Figure 2

Analysis of protein expression in undifferentiated and differentiated HOG cells. (A) Western blot analyses of myelin basic protein (MBP) (polyclonal), myelin-oligodendrocyte glycoprotein (MOG), CNPase, MBP (monoclonal), class III β-tubulin (TUBB3), proteolipid protein 1 (PLP1) and paraoxonase 2 (PON2) in (+) human motor cortex, (1) undifferentiated HOG cells, (2) differentiated HOG cells following incubation in N2.1 medium, (3) N2.2 medium or (4) T3 medium (normalized to GAPDH expression). Western blot analysis was performed for two independent experiments (n = 2). (B) Example images of immunocytochemistry for CNPase and TUBB3 in undifferentiated HOG cells and HOG cells differentiated with N2.1 medium or N2.2 medium. Scale bar = 50 µm.