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. 2019 Aug 21;8(9):948. doi: 10.3390/cells8090948

Figure 6.

Figure 6

ERK pathways are involved in ADAM9 and CTSS regulation by fisetin. (A,B) 786-O and Caki-1 cells were pre-treated with UO126 (5 μM) for 2 h and then incubated with or without fisetin (40 μM) for 24 h. Cell lysates were subjected to Western blotting to determine the expression of ADAM9, CTSB, CTSS, p-ERK, and t-ERK proteins. β-actin was used as an internal control for protein, with equal loading. (C,D) Then cells were assessed for the abilities of migration and invasion. Data are presented as the mean ± SE of at least three independent experiments. ** p < 0.01 compared with the untreated control (0 μM). # p < 0.01, compared with fisetin treatment alone.