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. 2019 Sep 15;8(9):1089. doi: 10.3390/cells8091089

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fisetin modulates psoriasis-like features in the 3D full-thickness reconstituted human skin model of psoriasis (FTRHSP). Immunofluorescence staining was performed on FTRHSP sections by incubating with primary antibody against targets overnight at 4 °C followed by incubation with specific Alexa Flour 488-labeled secondary antibodies for 2 h at room temperature in the dark. Samples were mounted in mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) and analyzed microscopically. Immunofluorescence staining analysis of differential staining showing the protein expressions of early (K10) and late (involucrin) differentiation markers in control RHSE and FTRHSP under different treatment conditions. Stainings are shown in red and green, respectively, and DAPI in blue and results are representative images of two independent experiments each performed in quadruplicate in comparison to the control and treated FTRHSP. Scale bar = 20 μm.