Table 2.
Validation of new Aspergillus fumigatus MAT LAMP assays by screening of isolates from diverse environmental sources and geographic localities
| Isolate | Source | Origin | MAT type (multiplex PCR)[Link] | LAMP detection time (min) | |
|---|---|---|---|---|---|
| MAT1‐1 assay | MAT1‐2 assay | ||||
| 47‐255 | Clinical | Europe | MAT1‐1 | 10‐11 | Negative |
| 47‐257 | Clinical | Europe | MAT1‐1 | 9‐10 | Negative |
| 47‐258 | Clinical | Europe | MAT1‐1 | 9‐10 | Negative |
| 47‐2 | Clinical | North America | MAT1‐1 | 9‐10 | Negative |
| C6‐UT1 | Food | Asia | MAT1‐1 | 15‐16 | Negative |
| C1‐2‐UT3 | Food | South America | MAT1‐1 | 9‐10 | Negative |
| C3‐UT1 | Food | South America | MAT1‐1 | 9‐10 | Negative |
| C3‐UT3 | Food | South America | MAT1‐1 | 9‐10 | Negative |
| O5‐5 | Plant | Africa | MAT1‐1 | 9‐10 | Negative |
| 18‐C6‐9 | Plant | Europe | MAT1‐1 | 9‐10 | Negative |
| 18‐C7‐8 | Plant | Europe | MAT1‐1 | 8‐9 | Negative |
| O9‐8 | Plant | Europe | MAT1‐1 | 12‐13 | Negative |
| T4‐1 | Plant | Europe | MAT1‐1 | 15‐16 | Negative |
| G4‐1 | Plant | South America | MAT1‐1 | 8‐9 | Negative |
| O10‐1 | Plant | South America | MAT1‐1 | 19‐20 | Negative |
| 1‐2.2‐B1 | Soil | Europe | MAT1‐1 | 9‐10 | Negative |
| 1‐2.2‐B2 | Soil | Europe | MAT1‐1 | 9‐10 | Negative |
| STNL1‐B1 | Soil | Europe | MAT1‐1 | 9‐10 | Negative |
| STNL1‐A8 | Soil | Europe | MAT1‐1 | 9‐10 | Negative |
| SWG1‐A9 | Soil | Europe | MAT1‐1 | 12‐13 | Negative |
| BKCb‐1 | Air | Europe | MAT1‐2 | Negative | 9‐10 |
| 47‐246 | Clinical | Europe | MAT1‐2 | Negative | 8‐9 |
| Af65 | Clinical | Europe | MAT1‐2 | Negative | 10‐11 |
| Af293 | Clinical | Europe | MAT1‐2 | Negative | 9‐10 |
| C5‐T8 | Food | Africa | MAT1‐2 | Negative | 9‐10 |
| 15‐37‐1 | Food | Asia | MAT1‐2 | Negative | 9‐10 |
| C1‐1‐T3 | Food | South America | MAT1‐2 | Negative | 9‐10 |
| C7‐T2 | Food | South America | MAT1‐2 | Negative | 9‐10 |
| C7‐UT1 | Food | South America | MAT1‐2 | Negative | 8‐9 |
| G2‐2 | Plant | Europe | MAT1‐2 | Negative | 7‐8 |
| SWF5‐C6 | Soil | Europe | MAT1‐2 | Negative | 7‐8 |
| PG1‐5 | Soil | Europe | MAT1‐2 | Negative | 9‐10 |
| WSN19‐3 | Soil | Europe | MAT1‐2 | Negative | 9‐10 |
| SWUK5‐A9 | Soil | Europe | MAT1‐2 | Negative | 8‐9 |
Determined by mating multiplex PCR assay.2