ACSL4 mRNA expression in various tissues under different physiological conditions. (A) mRNA was isolated from various tissues of 16- to 24-wk-old male and female mice (n = 3 to 6), and levels of ACSL4 mRNA were analyzed using RT-PCR. (B) Male mice that were 16 to 24 wk old were injected with ACTH or carrier, and tissues were collected 1 h later for analysis of ACSL4 mRNA levels (n = 3 to 6). (C) Female mice that were 16 to 24 wk old were injected with ACTH or carrier, and various tissues were collected 1 h later for analysis of ACSL4 mRNA levels (n = 3 to 6). Data are expressed as means ± SEM. *P < 0.05. BAT, brown adipose tissue; Epi, epididymal adipose tissue; Gastro-R; gastrocnemius muscle-red; Retro, retroperitoneal adipose tissue; SubQ, subcutaneous adipose tissue.