Wild type and mutant MVt and Vt proteins retain binding to F-actin. (A) Schematic representation of Vt and wild type and mutant MVt proteins. (B) Quantification of the Vt or MVt protein fractions present in the pellets, representing F-actin binding by individual proteins, using high-speed co-sedimentation. Concentration of wild type Vt, MVt and MVt mutant proteins is 10 μM. Error bars represent standard deviation (SD) (2 replicates for each system). Statistical significances with respect to Vt are indicated by non-significant (n.s.), p < 0.05 (*), p < 0.01 (**).