Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 1;10(5):e01395-19. doi: 10.1128/mBio.01395-19

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Chmiel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 3 — IMI impairs Duox pathway expression via the Imd pathway. (A and B) norpA⁷ (PLC-β^−/−) flies exposed to 10 μM IMI or vehicle (DMSO) for 5 days. (A) Duox gene expression data points are displayed as mean fold change (relative to RpLP0) of 8 biological replicates with 5 pooled female flies in each group. Error bars represent mean ± SD (Mann-Whitney test). (B) Whole-body H₂O₂ displayed as mean relative % of H₂O₂ ± SD (unpaired, two-tailed t test) from 7 biological replicates (each consisting of 3 flies). (C and D) R156 imd¹ (IMD^−/−) flies exposed to 10 μM IMI or vehicle (DMSO) for 5 days. (C) Duox gene expression data points are displayed as mean fold change (relative to RpLP0) from 7 biological replicates with 5 pooled female flies in each group. Error bars represent mean ± SD (Mann-Whitney test). (D) Whole-body H₂O₂ displayed as mean relative % of H₂O₂ ± SD (unpaired, two-tailed t test) from 10 biological replicates (each consisting of 3 flies). (E and F) PGRP-LE¹¹² (PGRP-LE^−/−) flies exposed to 10 μM IMI or vehicle (DMSO) for 5 days. (E) Duox gene expression data points are displayed as mean fold change (relative to RpLP0) from 8 biological replicates with 5 pooled female flies in each group. Error bars represent mean ± SD (Mann-Whitney test). (F) Whole-body H₂O₂ displayed as mean relative % of H₂O₂ ± SD (unpaired, two-tailed t test) from 12 biological replicates (each consisting of 3 flies). (G and H) PGRP-LC^Δ^E (PGRP-LC^−/−) flies exposed to 10 μM IMI or vehicle (DMSO) for 5 days. (G) Duox gene expression data points are displayed as mean fold change (relative to RpLP0) from 8 biological replicates with 5 pooled female flies in each group. Error bars represent mean ± SD (Mann-Whitney test). (H) Whole-body H₂O₂ displayed as mean relative % of H₂O₂ ± SD (unpaired, two-tailed t test) from 10 biological replicates (each consisting of 3 flies). In box plot diagrams, boxes represent first and third quartile values, while black lines denote medians. Whiskers encompass maximum and minimum values. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.