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Activation of AMPK in skeletal muscle following 2-AA treatment. (Top) Representative immunoblots of pAMPKβ1 (Ser182) in skeletal muscle 1 day, 2 days, and 4 days after 2-AA treatment and 2-AA-plus-NAC treatment. AMPKβ1/2 was used as a control. (Bottom) Histograms show the relative expression levels of proteins. n = 5 in each group; data represent means ± SD. P < 0.05, one-way ANOVA.
