Extended Data Figure 5 |. FPR1−/− cells migrate towards chemoattractants other than formylated peptides and differentiated HL-60 cells migrate towards Y. pestis.
a, Numbers of migrating immune cells were quantified in a transwell assay primed with mock, 10 nM fMLF, 10 nM LTB4 or 100 ng ml−1 KC (CXCL1) for U937, FPR1−/− and FPR1−/− (pFPR1) cells. b, Numbers of migrating HL-60 cells were quantified in a transwell assay primed with mock, Y. pestis KIM D27 (WT) or KLD29 (ΔlcrV) (107 CFU/ml). Chemotaxis toward fMLF is shown as a control. c, Increasing concentrations of LcrVS228 (10−1–103 ng/ml) were added to the transwell assay and number of migrating HL-60 cells recorded. Error bars represent the s.e.m. (n = 3 biological replicates); one-way ANOVA with Bonferroni Correction was used to identify significant differences: ***, P<0.001; **, P <0.01; *, P<0.05; ns, not significant. A representative of three independent experiments is shown.