Extended Data Figure 4 |. Screening 45 monoclonal antibodies for inhibition of Y. pestis T3SS into human neutrophils.

Human neutrophils were stained with the β-lactamase substrate CCF2-AM (green fluorescence), and infected with wild-type Y. pestis KIM D27 (pMM83). Translocation of YopM-Bla into neutrophils results in CCF2-AM cleavage (blue fluorescence) allowing for quantification of percent of stained cells injected with T3SS effector in the absence (Mock) or presence of specific monoclonal antibody. Error bars represent the s.e.m. (n = 3 biological replicates). A representative of three independent experiments is shown.