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Application of cyclopamine at HH20 causes loss of Cyclin D2 expression in the polarising region at 8 hr (a, b, n = 4/4 in each experiment) and 30 hr (c, d n = 4/4 in each experiment). Although p27kip1 expression is unaffected at 8 hr (e, f, n = 6/6 in each experiment) it is undetectable at 30 hr (g, h, n = 5/5 in each experiment). Scale bars: a, b, e, f – 150 μm; c, d, g, h – 300 μm.
