Table 2.

GnRH-Induced Increase of Srxn1 Gene Expression of in LβT2 Cells

	GnRH Pulse Concentration, 10 nM		GnRH Pulse Concentration, 100 nM
GnRH Pulse Interval, min	Fold Response	P	Fold Response	P
240	0.97	NS	2.62	<0.001
120	2.90	<0.001	6.07	<0.001
60	7.34	<0.001	11.73	<0.001
30	10.40	<0.001	15.92	<0.001
Tonic	ND	ND	21.70	<0.001

Extract of Srxn1 (Npn3) expression measurements as reported by Lawson et al. (2) and Mistry et al. (3). Perifusion cultures of LβT2 cells were pulsed at 10 nM and 100 nM peak GnRH for 4 h at the intervals indicated. Cells were harvested and processed for analysis by Affymetrix MU74AV2 expression array. Resultant signal data were evaluated using VAMPIRE Bayesian variance modeling and significance based on α_bonf < 0.05, as described previously (2). Srxn1/Npn3 expression is shown as fold change relative to vehicle control and exhibits significant increases based on frequency and amplitude of stimulation. Data were extracted from the analyzed data set reported by Lawson et al. (2). Significance was determined by difference from the corresponding vehicle treatment as ascertained by Student t test.

Abbreviations: ND, no data; NS, not significant.