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ERBB2 protein expression in MCF-7 cells. Cells were treated with media (untreated), carbonate apatite, free ERBB2 siRNA, CA/ERBB2 siRNA, CA/Doc/ERBB2, CA/Pac/ERBB2, CA/Doc, and CA/Pac. Ingredients include 3 mM of CaCl2, 1 nM siRNA, and 1 nM drug. Cellular lysates were run in SDS-PAGE and transferred into PVDF membrane and incubated with primary antibodies raised in rabbit against ERBB2. HRP-conjugated goat anti-rabbit secondary antibody was used to detect the chemiluminescent signals.
