Pro-CO factors associate with SYP-1 stretches and between sister chromatid pairs in rec-8 mutants. (A) Immunolocalization of SYP-1 and GFP::COSA-1 in fields of nuclei from the late pachytene regions of wild-type, rec-8(ok978), and rec-8; syp-2 germ lines. Average number of COSA-1 foci per nucleus and SD is labeled on the image for each genotype. Bar, 5 μm. (B) Stacked bar graph showing percentages of nuclei with indicated numbers of GFP::COSA-1 foci in late pachytene for wild-type, rec-8(ok978), and rec-8; syp-2 (Table S1). Number of late pachytene nuclei scored for COSA-1 foci: wild-type, n = 505; rec-8(ok978), n = 245; and rec-8; syp-2, n = 204. (C) Immunolocalization of GFP::COSA-1 in DAPI-stained diplotene and diakinesis bivalents from rec-8(ok978) germ lines. CO, crossover.