Table 2. pol30-6 and pol30-79 cause high rates of mitotic recombination and gene conversion in diploids.
% Dead:alive tetrads | ||||||||
---|---|---|---|---|---|---|---|---|
Alleles | 0:4 | 1:3 | 2:2 | 3:1 | 4:0 | % Inviable spores | Total tetrads | Strain numbers |
POL30/POL30 | 85 | 5 | 5 | 5 | 0 | 8 | 20 | JRY11159 |
POL30/pol30∆ | 0 | 0 | 93 | 5 | 3 | 52 | 40 | JRY11745, JRY11746 |
pol30-6/pol30-6 | 30 | 20 | 36 | 11 | 4 | 34 | 56 | JRY11686, JRY11714, JRY11715 |
pol30-6/pol30∆ | 0 | 0 | 50 | 20 | 30 | 63 | 40 | JRY11747, JRY11748 |
pol30-8/pol30-8 | 90 | 10 | 0 | 0 | 0 | 3 | 20 | JRY11687 |
pol30-8/pol30∆ | 0 | 0 | 80 | 20 | 0 | 55 | 40 | JRY11749, JRY11750 |
pol30-79/pol30-79 | 20 | 11 | 36 | 20 | 14 | 46 | 56 | JRY11688, JRY11716, JRY11717 |
pol30-79/pol30∆ | 0 | 0 | 40 | 45 | 16 | 65 | 38 | JRY11751, JRY11752 |
pol30-6/pol30-79 | 88 | 12 | 0 | 0 | 0 | 3 | 42 | JRY11657, JRY11685 |
pol30-6/pol30-8 | 61 | 22 | 11 | 5 | 0 | 15 | 18 | JRY11656 |
pol30-79/pol30-8 | 75 | 5 | 15 | 5 | 0 | 13 | 20 | JRY11658 |
Spore inviability measurements. At least one, and up to three, independent diploids were sporulated and their tetrads dissected from each of the indicated genotypes. After 2–3 days of growth, tetrads were scored for inviability. The first five columns are the percentages of each combination of dead:alive spores per tetrad.