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. Author manuscript; available in PMC: 2019 Oct 8.
Published in final edited form as: Immunometabolism. 2019 Sep 25;1:e190014. doi: 10.20900/immunometab20190014

Figure 3. NK1.1 activating receptor ligation facilitates IL2-mediated metabolic responses in NK cells.

Figure 3

Cultured NK cells (6 days in IL15 10 ng/mL) were purified and stimulated with a plate bound α-NK1.1 antibody (10 μg/mL) in media supplemented with low dose IL15 (5 ng/mL) ± IL2 (20 ng/mL) as indicated, with IL2 (20 ng/mL) plus IL12 (10 ng/mL) or left unstimulated (IL15 - 5 ng/mL). Cells were analysed for rates of glycolysis (a,f,g) and OXPHOS (b,d,e) using the seahorse extracellular flux analyser or by flow cytometry for the expression of CD25 (c), CD71 (h) or CD98 (i). Data is representative (a–d,f) or mean ± SEM (a,b,e, g–i) of 4–5 independent experiments. Data was analyzed using a one way ANOVA and tukey post test (* p < 0.05, ** p < 0.01, *** p < 0.001, ns non-significant).