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. 2019 Sep 17;11(9):869. doi: 10.3390/v11090869

Figure 2.

Figure 2

Map for the synthetic plasmid pcIpR-GOI-timm expression vector, used to clone any Gene of Interest (pink), e.g., the D-fusion gene shown, with ATG for GOI at base 842 of plasmid. In the versions of this plasmid used herein where the gene of interest (GOI) represents D or D-fusions, the wild type sequence for D was synthetically optimized in 55 of its 110 codons [26,27] to improve translation efficiency. This version of D is termed Dcoe and the protein product is called gpDcoe (representing codon enhanced). The sequence of Dcoe is provided in the supplement to reference [26], where Dcoe was fused to a linker and four regions of the porcine Circovirus 2 capsid gene, i.e., on plasmid pcIpR-D-CAP-timm. Base assignments after 842 depend on the size of the GOI insertion and so are not shown. All versions of the pcIpR-GOP-timm expression plasmid [26,28,29,30,31] include the strong rightward lambda promoter pR. The expression of a D or D-fusion gene cloned as GOI into pcIpR-GOI-timm is negatively regulated.